Observation and measurement of the exteriorized spinotrapezius preparation were carried out with an intravital microscope (Axio Imager A2m; Carl Zeiss, Göttingen, Germany) configured for trans-illumination through a 20×/0.8 objective (Plan-APOCHROMAT; Zeiss, Jena, Germany) and custom modified for PQM. Trans-illumination was used to select measurement sites, establish appropriate focal planes, and verify flow conditions. Measurements of arteriolar diameter in the spinotrapezius muscle were made using a 10×/0.25 objective (N-Achroplan; Zeiss). The objective was focused in the diametral plane and the image displayed on an ultra-high definition screen (XBR43X830C; Sony, Tokyo, Japan). Images of the microvasculature were captured in real-time by a color CMOS camera (ACE ACA2040–90UC; Basler, Berlin, Germany) and stored digitally. Internal (luminal) vessel diameters for second-order arterioles were measured by converting from pixels to micrometers using a calibration based on pixelization of a standard microscope micrometer (Stage Micrometer; Zeiss). Arterioles between 40 and 80 μm were selected for assessment of vasoactivity.
Xbr43x830c
Manufactured by Sony
Sourced in Japan
The XBR43X830C is a 43-inch 4K HDR LED television manufactured by Sony. It features a 3840 x 2160 resolution, HDR, and a standard refresh rate. The device supports HDMI and USB connectivity.
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2 protocols using xbr43x830c
1
Intravital Microscopy of Spinotrapezius
2
Intravital Microscopy of Spinotrapezius Arterioles
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Observation and measurement of the exteriorized spinotrapezius preparation were carried out with an intravital microscope (Axioimager2m, Carl Zeiss, Jena, Germany) configured for trans-illumination through a 20X/0.8 objective (Plan-APOCHROMATE, Zeiss) and custom modified for PQM. Trans-illumination was used to select measurement sites, establish appropriate focal planes, and verify flow conditions. Measurements of arteriolar diameter in the spinotrapezius muscle were made using a 10X/0.25 objective (N-Achroplan, Zeiss). The objective was focused in the diametral plane and the image displayed on an ultra-high definition screen (XBR43X830C, Sony, Tokyo, Japan). Images of the microvasculature were captured in real time by a color CMOS camera (ACE ACA2040-90UC, Basler AG, Ahrensburg, Germany) and stored digitally. Internal (luminal) vessel diameters for second-order arterioles were measured by converting from pixels to μm using a calibration based on pixelization of a standard microscope micrometer (Stage Micrometer, Zeiss). Arterioles <80 micrometers (μm) were selected for assessment of vasoactivity and divided into two groups: 40 μm to 80 μm as measured in our previous study; and 10 μm to 40 μm, which includes precapillary arterioles with less vascular smooth muscle (21) .
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