Mouse embryonic stem (ES) cells used for chromatin immunoprecipitation (ChIP) experiments were derived and cultivated as described previously (Mohn et al., 2008 (link)). ES cells differentiation was carried out as described previously (Bibel et al., 2007 (link)). Briefly, embryonic stem cells were cultured feeder-free for three to five passages after which LIF was withdrawn to allow formation of cellular aggregates in liquid culture. After 4 days, retinoic acid (RA) was added to induce neuronal progenitor (NP) formation for another 4 days before chromatin isolation. All the other mammalian cell lines were cultured in DMEM (PAA, GE Healthcare Life Sciences, Sweden) medium supplemented with 10% FCS, 2 mM L-glutamine, 100 U/ml Penicillin and 0.1 mg/ml Streptomycin. For the HDAC inhibitors treatment either Na-butyrate (10 mM, overnight) or Trichostatin A (TSA, 0.2 µM, 48 hr) were used.
Dmem paa
Manufactured by GE Healthcare
Sourced in Sweden
DMEM (Dulbecco's Modified Eagle Medium) is a cell culture medium developed by GE Healthcare. It is a basal medium that provides essential nutrients, amino acids, vitamins, and other components required for the growth and maintenance of various cell types in in vitro cell culture applications.
Automatically generated - may contain errors
Lab products found in correlation
Pen strep, by Thermo Fisher Scientific (1 mentions)
Pen strep, by Merck Group (1 mentions)
Mc3t3 e1 cell line, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Opti mem, by Thermo Fisher Scientific (1 mentions)
Glutamine, by Merck Group (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
C2c12 cell line, by Merck Group (1 mentions)
2 protocols using dmem paa
1
Mouse ES Cells ChIP-seq Protocol
2
Cell Culture Protocols for Osteoblast and Myoblast Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The MC3T3-E1 cell line was obtained from the American Type Culture Collection (LGC Standards S.r.L., Sesto S. Giovanni, MI, Italy) and cultured in Dulbecco's modified Eagle'a medium (DMEM, PAA; GE Healthcare, Uppsala, Sweden), 10% fetal bovine serum (FBS; Gibco, ThermoFisher, Waltham, MA, USA), 1% penicillin and streptomycin (Penstrep; Sigma-Aldrich, St. Louis, MO, USA) and 1% glutamine (Sigma-Aldrich, St. Louis, MO, USA) as subculture medium. For gene expression assays, cells were plated on polished or SLA surfaces at the density of 30,000 cells/well in 24-well plates, in triplicate. After 24 hours, the subculture medium was replaced with subculture medium added with 250 μM ascorbic acid (Sigma Chemicals, St. Louis, MO, USA).
The C2C12 cell line was obtained from the European Catalog of Cell Cultures (Health Protection Agency Culture Collections, Salisbury, UK) and grown in subculture medium. For reporter assays, C2C12 cells were plated on titanium surfaces in OptiMEM (Invitrogen, San Giuliano Milanese, MI, Italy), 5% FBS, 1% Penstrep at the density of 125,000 cells/well, and the cells were assayed after 24 hours. C2C12 is a popular cell line to study Wnt canonical signaling because of the abundance of molecular machinery for this pathway.
The C2C12 cell line was obtained from the European Catalog of Cell Cultures (Health Protection Agency Culture Collections, Salisbury, UK) and grown in subculture medium. For reporter assays, C2C12 cells were plated on titanium surfaces in OptiMEM (Invitrogen, San Giuliano Milanese, MI, Italy), 5% FBS, 1% Penstrep at the density of 125,000 cells/well, and the cells were assayed after 24 hours. C2C12 is a popular cell line to study Wnt canonical signaling because of the abundance of molecular machinery for this pathway.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!