Nitrocellulose paper (#1620177), Laemmle sample buffer (#1610737) and HRP substrate (IMMUN-STAR #1705040) were from Bio-Rad (Hercules, CA, USA). The Bicinchonic Acid Protein Assay Kit (#BCA1; B9643) and protein inhibitor cocktail (#P2714) were from Sigma Chemical Co. (St. Louis, MO). The anti-heat shock protein 70 (HSP-70) (rabbit polyclonal, SC-71060R), anti-C/EBP-homologous protein (CHOP) (rabbit polyclonal, SC-575) and anti-X-box binding protein 1 (XBP-1) (M186) (rabbit polyclonal, SC-7176) antibodies were from Santa Cruz Biotechnology, Inc. (Dallas, Texas, USA). The anti-glucose regulating protein 78 (BIP) (rabbit polyclonal, #C50B12) antibody was from Cell Signaling Technology (Beverly, MA, USA). Routine reagents were purchased from Sigma Chemical Co. (St. Louis, MO, USA).
Anti c ebp homologous protein chop
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti-C/EBP-homologous protein (CHOP) is a laboratory product used for research purposes. CHOP is a transcription factor that plays a role in the cellular response to endoplasmic reticulum stress. This product can be used to study CHOP and its functions in various experimental systems.
Automatically generated - may contain errors
Lab products found in correlation
Anti caspase 12, by Cell Signaling Technology (2 mentions)
Protein inhibitor cocktail, by Merck Group (1 mentions)
Nitrocellulose paper, by Bio-Rad (1 mentions)
Hsp70, by Santa Cruz Biotechnology (1 mentions)
Xbp1 m 186, by Santa Cruz Biotechnology (1 mentions)
Anti notch1, by Abcam (1 mentions)
Anti hes1, by Abcam (1 mentions)
Anti gapdh, by Thermo Fisher Scientific (1 mentions)
Anti atf6, by Santa Cruz Biotechnology (1 mentions)
Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Anti bcl 2 associated x protein bax, by Santa Cruz Biotechnology (1 mentions)
Horseradish peroxidase conjugated goat anti rabbit secondary antibody, by Thermo Fisher Scientific (1 mentions)
Anti pgc 1α, by Abcam (1 mentions)
Anti bip, by Cell Signaling Technology (1 mentions)
Anti sod1, by Enzo Life Sciences (1 mentions)
Anti akt1 b 1, by Santa Cruz Biotechnology (1 mentions)
Anti mac2, by American Type Culture Collection (1 mentions)
Anti actin, by Merck Group (1 mentions)
Rhodamine conjugated α bungarotoxin, by Thermo Fisher Scientific (1 mentions)
Anti ha, by Merck Group (1 mentions)
3 protocols using anti c ebp homologous protein chop
1
Endoplasmic Reticulum Stress Protein Analysis
2
Western Blot Analysis of Kidney Protein Markers
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Equal amounts of protein samples from mouse kidney were separated by SDS-PAGE, transferred onto polyvinylidene fluoride membranes by electroblotting and incubated at 4 °C overnight with primary antibodies (anti-PGC-1α (Abcam, Cambridge, UK), anti-ATF6 (Santa Cruz Biotechnology, USA), anti-C/EBP homologous protein (CHOP, Santa Cruz Biotechnology, USA), anti- phospho-IRE1α (p- IRE1α, Invitrogen, USA), anti-phospho-PERK (p-PERK, Invitrogen, USA), anti-cleaved Poly (ADP-ribose) polymerase 1 (c-PARP1, Santa Cruz Biotechnology, USA), anti-mitofusin 2 (Mfn2, Abcam, Cambridge, UK), anti-apoptotic protein B-cell lymphoma 2 (Bcl2, Santa Cruz Biotechnology, USA), anti-Bcl2-associated X protein (Bax, Santa Cruz Biotechnology, USA), anti-cleaved caspase 3 (Cell Signaling Technology, USA), anti-caspase 12 (Cell Signaling Technology, USA), anti-Notch1 (Abcam, Cambridge, UK), anti-Hes1(Abcam, Cambridge, UK) and anti-GAPDH (Invitrogen, USA)). Next, the membranes were incubated with horseradish peroxidase–conjugated goat anti–rabbit secondary antibody (Invitrogen, USA) at 25 °C for 1.5 h. The chemiluminescent bands were visualized using a chemiluminescence detection system and quantified using ImageJ software.
3
Comprehensive Antibody Staining Protocol
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The following antibodies were used in this study: anti-FLAG (M2, Sigma Aldrich, St. Louis, MO), anti-HA (H6908, Sigma;3F10, Roche, Mannheim, Germany), anti-SOD1 (SOD100, Enzo Life Science, Farmingdale, NY; C17, Santa Cruz Biotechnology, Santa Cruz, CA), anti-chromogranin B (26102, QED Bioscience, San Diego, CA; PA1-10839, Thermo, Rockford, IL), B8H10 (33), C4F6 (37), anti-TGN38 (M290, Santa Cruz), anti-synaptophysin (D35E4, Cell signaling technology, Danvers, MA), anti- synaptotagmin V (46, Santa Cruz), anti-Akt1 (B1, Santa Cruz), anti-Mac2 (hybridoma from ATCC, Rockville, MD, USA), anti-glial fibrillary acidic protein (GFAP) (GA5, Cell signaling), anti-choline acetyltransferase (ChAT) (AB144P, Millipore, Billerica, MA), anti-neuronal nuclear antigen (NeuN) (MAB5294, Millipore), anti-actin (Millipore), anti-human protein gene product 9.5 (PGP9.5) (7863-0504, AbD Serotec, Raleigh, NC), anti-vesicular acetylcholine transport (VAChT) (Milipore), rhodamine conjugated α-Bungarotoxin (Invitrogen, Carlsbad, CA), anti-Bip (3177, Cell Signaling; ab21685, abcam, Cambridge, MA), anti-phospho-protein kinase-like endoplasmic reticulum kinase (PERK) (16F8, Cell Signaling), anti-C/EBP-homologous protein (CHOP) (F168, Santa Cruz), anti-caspase 12 (2202, Cell Signaling), SMI32 (Covance, Princeton, NJ), anti-SRY (E19, Santa Cruz).
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