To observe the effects of the acetone extract on shape and adherence of the MDA-MB-231 cells, cells in 96-well plates were observed under a phase contrast microscope (OPTIKA, Italy) using 40X magnifications and photographed for all time points mentioned in the above experimental design. The cells were photographed under the inverted microscope using Optika vision lite software. Pictures were saved in JPG format for further study and analysis.
Vision lite software
Manufactured by Optika
Sourced in Italy
Vision Lite software is a data analysis tool used to process visual data from optical instruments. The software's core function is to enable users to capture, process, and analyze visual information from various sources.
Automatically generated - may contain errors
3 protocols using vision lite software
1
Acetone Extract Effects on MDA-MB-231 Cell Morphology
2
Microgel Structural Characterization
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Microgels recovered in collecting baths were observed using an optical microscope (Optika, Italy). The size of microgels (N = 30) was determined using Optika Vision Lite software.
Scanning electron microscopy SEM observations on freeze-dried microgels were performed using JEOL JSM 7600F instrument (JEOL, Japan) working at an accelerating voltage of 5KV.
Images were collected by the Everhart-Thornley secondary electron detector of the chamber, far enough of the lens to ensure good depth of field.
Scanning electron microscopy SEM observations on freeze-dried microgels were performed using JEOL JSM 7600F instrument (JEOL, Japan) working at an accelerating voltage of 5KV.
Images were collected by the Everhart-Thornley secondary electron detector of the chamber, far enough of the lens to ensure good depth of field.
3
Ileal Morphology and Goblet Cell Analysis
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In Experiment 2, ileal tissue samples were cut into two longitudinal sections and embedded in paraffin. Two slides were made from each sample to obtain ileal morphology measurements (Hematoxylin and Eosin stain) and goblet cell (GC) categorization (Alcian Blue/ Periodic Acid-Schiff stain). An Optika B-290TB digital microscope (Bergamo, Italy) was used to observe slides, and an HDCE-X3 digital camera with Optika Vision Lite software was used to capture the images. Well-oriented 8-10 villi and crypts per section were used to measure villi length, width, and crypt depth. Villi length was considered as the length from the tip of a villus to the villus-crypt junction. The villi width was measured at the middle of the villus height. The depth of the invagination between adjacent villi was considered as the crypt depth. Goblet cells were counted around the perimeter of 8-10 well-oriented villi per section, and the three categories of GC were identified, acidic mucin-producing GC (stained in blue), neutral mucinproducing GC (stained in magenta) and mixed mucin-producing GC (stained in purple) [40] (link).
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