Reboxetine mesylate hydrate

Ketamine hydrochloride (Vet One, MWI Animal Health, Boise, ID, USA), fluoxetine hydrochloride (Sigma Aldrich, St. Louis, MO, USA) and reboxetine mesylate hydrate (Sigma Aldrich, St. Louis, MO, USA), were individually dissolved in sterile saline (0.9% NaCl solution, Hospira, Mountainside Medical Equipment, Marcy, NY, USA) and administered via intraperitoneal injection (i.p.) at 10 mg kg⁻¹ and a volume of 5 mL kg⁻¹ body weight. Escitalopram oxalate (Sigma Aldrich, St. Louis, MO, USA) was prepared and administered following the same method at the following doses: 1 mg kg⁻¹, 3 mg kg⁻¹, 10 mg kg⁻¹ and 30 mg kg⁻¹. Urethane (Sigma Aldrich, St. Louis, MO, USA) was dissolved in sterile saline at 25% w/v and administered at 7 μLg⁻¹ mouse body weight for surgical anesthesia. Post-calibration solutions for FSCAV were prepared by dissolving serotonin hydrochloride (Sigma–Aldrich Co., St. Louis, MO, United States) in Tris buffer to make solution concentrations of 10, 25, 50, and 100 nM. Tris buffer consisted of: 15 mM H₂NC(CH₂OH)₂•HCl, 140 mM NaCl, 3.25 mM KCl, 1.2 mM CaCl₂, 1.25 mM NaH₂PO₄•H₂O, 1.2 mM MgCl₂, and 2.0 mM Na₂SO₄ (Sigma–Aldrich Co., St. Louis, MO, United States) in deionized water; the pH adjusted to 7.4 (± 0.03).

Drugs: Methiopropamine hydrochloride (HCl) (mol. wt. 191.7) was purchased from Cayman Chemicals (Middlesex, UK) and (+)methamphetamine HCl (mol. wt. 185.7) was purchased from Chiron AS (Trondheim, Norway). Chemicals: 5-HT HCl, L-NE HCl, 5-HIAA HCl, 3-MT HCl, ephedrine-D₃, sodium fluoride, methanol (LC-MS Chromasolv^®), ammonium formate, benztropine mesylate, reboxetine mesylate hydrate, fluoxetine HCl and adenosine 5′-triphosphate disodium salt hydrate were purchased from Sigma-Aldrich (Oslo, Norway). DA HCl and DOPAC were purchased from Fluka (Buchs, Switzerland). DA-¹³C₆ HCl, 5-HT-D₄ HCl, 5-HIAA-D₅, methamphetamine-¹³C₆ and nor-methiopropamine were purchased from Chiron AS. Di-sodiumtetraborate-10-hydrate and ethyl acetate were purchased from Chemi-Teknik AS (Oslo, Norway). Acetic acid and formic acid were purchased from VWR International AS (Oslo, Norway). Sodium hydroxide and n-heptane were purchased from Merck Millipore (Oslo, Norway). Dihydroxyphenylethylamine 3,4-[ring-2,5,6-³H]-(DA) ([³H]DA), NE HCl DL-[7-³H(N)] ([³H]NE), 5-HT [³H(G)] ([³H]5-HT) and UltimaGold^TM liquid scintillation cocktail were purchased form from PerkinElmer Inc. (Oslo, Norway). HEPES was purchased from Thermo Fischer Scientific (Oslo, Norway) and sodium heparin was purchased from Leo Pharma (Oslo, Norway).