Poly l ornithine hydrobromide plo

Manufactured by Merck Group

Sourced in United States

Poly-L-ornithine hydrobromide (PLO) is a synthetic amino acid polymer commonly used as a cell culture coating agent. It functions by promoting cell adhesion and growth on various surfaces. PLO is water-soluble and can be applied to laboratory equipment and cell culture vessels to enhance cellular attachment and proliferation.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Poly-L-ornithine (PLO, Poly-L-ornithine hydrobromide Poly-L-ornithine PLO/L Poly-ornithine L-ornithine

5 protocols using poly l ornithine hydrobromide plo

Polymer-Supplemented Cell Culture Media

Check if the same lab product or an alternative is used in the 5 most similar protocols

High-molecular-weight (M.W.) soluble polymers and their final concentration in culture
medium (CiCM) were as follows: Poly-ethylene glycol (PEG) [M.W. 15000-25000, nacalai
tesque]: CiCM 0.5-1 mg/ml; polyvinyl alcohol (PVA) [M.W. 146000-186000, Sigma-Aldrich]:
CiCM 0.5-1 mg/ml; polyvinylpyrrolidone (PVP) [M.W. 360000, Sigma-Aldrich]: CiCM 1 mg/ml;
dextran (Dex) [M.W. 190000-230000, nacalai tesque]: CiCM 1 mg/ml; poly-γ-glutamic acid
(PGA) [M.W. 200000-500000, Wako Pure Chemical industry]: CiCM 1 mg/ml; chondroitin sulfate
C sodium salt (CSC) [M.W. 40000-80000,Wako Pure Chemical industry]: CiCM 0.5 mg/ml;
hyaluronic acid sodium salt (HRL) [M.W. not determined, Wako Pure Chemical industry]: CiCM
0.5 mg/ml; chondroitin sulfate A sodium salt (CSA) [M.W. 40000-80000, Sigma-Aldrich]: CiCM
0.5 mg/ml; dermatan sulfate sodium salt (DS) [M.W. 85000~100000, Tokyo Chemical Industry]:
CiCM 0.5 mg/ml; poly-L-lysine hydrobromide (PLL) [M.W. 30000-70000, Sigma-Aldrich] CiCM
5-50 μg/ml; poly-L-ornithine hydrobromide (PLO) [M.W. 30000-70000, Sigma-Aldrich]: CiCM
12.5 μg/ml; poly-D-lysine hydrobromide (PDL) [M.W. 30000-70000, Sigma-Aldrich]: CiCM 12.5
μg/ml; poly-L-histidine (PLH) [M.W. 5000-25000, Sigma-Aldrich]: CiCM 12.5 μg/ml.

Kagami Y., Kato H., Okada Y., Seto M, & Yamamoto K. (2021). Establishment of an ATLL cell line (YG-PLL) dependent on IL-2 and IL-4, which are replaced by OX40-ligand+ HK with poly-L-histidine and dermatan sulfate. Journal of Clinical and Experimental Hematopathology : JCEH, 61(3), 145-151.

+ Open protocol

+ Expand

Isolation and Culture of Primary Murine Cortical Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols

C57BL/6 pups of embryonic day 15 were used. Embryos were dissociated from amniotic sac, and fetuses were put into a sterile petri dish with HBSS. They were decapitated, and skull and meninges were removed on ice. Only cortex was collected in 50 ml tube with HBSS and washed 3 times with 20 ml of HBSS to clear the debris. The cortex was incubated in 5 ml HBSS containing 0.1% trypsin for 5 min in a 37℃ water bath (shaking every 1 min). Afterward, 5 ml FBS (1X volume) was added to the tube, and the cortex was dissociated by pipetting. The cells were collected using a cell strainer (40 μm) and centrifuge (800 g, 3 min). The supernatant was removed, and the cell pellet was resuspended in Neurobasal A medium (10888022, Gibco). 1×10⁴ cells were plated on 0.1 mg/ml Poly-L-Ornithine hydrobromide (PLO) (P3655, Sigma-Aldrich)-coated 24 well plates and incubated in the incubator (37℃, 5% CO₂). The neuron culture was maintained by replacing the half of medium with fresh medium: Neurobasal A medium+2% B27 (17504-044, Gibco)+1% Glutamax (35050-061, Thermofisher Scientific)+0.5% P/S (250 ul/50 ml) (SV30010, Hyclone) every 2~3 days.

Jang M.W., Kim T.Y., Sharma K., Kwon J., Yi E, & Lee C.J. (2021). A Deafness Associated Protein TMEM43 Interacts with KCNK3 (TASK-1) Two-pore Domain K+ (K2P) Channel in the Cochlea. Experimental Neurobiology, 30(5), 319-328.

+ Open protocol

+ Expand

PEGylated Polyornithine-Based Biomaterials

Check if the same lab product or an alternative is used in the 5 most similar protocols

Poly-l-ornithine hydrobromide (PLO, MW 44.9 kDa) and FD-4 (MW 3.4 kDa) were purchased from Sigma-Aldrich (St. Louis, MO, USA). α-Succinimidyloxy carbonyl-ω-methoxy polyoxyethylene (mPEG-NHS; SUNBRIGHT ME-100TS, MW 9.8 kDa) and α-mercaptoethyl-ω-methoxy polyoxyethylene (mPEG-SH; SUNBRIGHT ME-100SH; MW 9.2 kDa) were obtained from NOF Corporation (Tokyo, Japan). 2-Morpholinoethanesulfonic acid monohydrate (MES) and MTT were purchased from Dojindo Laboratories (Kumamoto, Japan). TNBS was obtained from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). The cell culture reagents and supplies were purchased from Thermo Fisher Scientific, Inc. (Waltham, MA, USA). All other reagents were of analytical reagent grade.

Kamiya Y., Yamaki T., Omori S., Uchida M., Ohtake K., Kimura M., Yamazaki H, & Natsume H. (2018). Improved Intranasal Retentivity and Transnasal Absorption Enhancement by PEGylated Poly-l-ornithine. Pharmaceuticals, 11(1), 9.

+ Open protocol

+ Expand

NESCs, APCs, and Astrocytes Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

For maintenance culture of NESCs and differentiation of APCs and astrocytes, 6-well plates were coated with Matrigel™ (1:40 in DMEM/F-12) and incubated for 30 min at 37 °C and 5% CO₂. Afterwards, unadsorbed Matrigel™ was aspirated and cells were directly seeded onto plates.
For NFκB translocation quantification, Western blot and transcriptome sample generation, 96-well plates were coated with 43 μg/mL poly-L-ornithine hydrobromide (PLO, Sigma-Aldrich, St. Louis, MO, USA) and 1 μg/mL fibronectin (Sigma-Aldrich, St. Louis, MO, USA) in Milli-Q H₂O. After incubation at 37 °C overnight, plates were washed three times with Milli-Q H₂O, dried and stored at 4 °C until further use.
For immunofluorescence staining of cells, cover slips with a diameter of 13 mm (Thermo Fisher Scientific, Waltham, MA, USA) were transferred into 24-well plates and coated with Matrigel™ 1:20 in DMEM/F-12 at 37 °C and 5% CO₂ for 30 min.

Spreng A.S., Brüll M., Leisner H., Suciu I, & Leist M. (2022). Distinct and Dynamic Transcriptome Adaptations of iPSC-Generated Astrocytes after Cytokine Stimulation. Cells, 11(17), 2644.

+ Open protocol

+ Expand

Culturing Neural Cell Lines with Aeruginol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dibutyryl cyclic adenosine monophosphate (dBcAMP), fibronectin, fetal calf serum (FCS), Hoechst H-33342, poly-L-ornithine hydrobromide (PLO), resazurin sodium salt, tetracycline, sodium pyruvate, and Triton-X100 were purchased from Sigma Aldrich (Steinheim, Germany). Chemicals and solvents used for extraction, purification and synthesis of aeruginol and derivatives were purchased from Sigma Aldrich (Steinheim, Germany). Nicotinamide-adenine-dinucleotide (NADH) disodium salt was purchase from Carl Roth GmbH + Co. KG (Karlsruhe, Germany). Recombinant human fibroblast growth factor 2 (FGF-2) and recombinant human glial cell derived neurotrophic factor (GDNF) were purchased from R&D Systems (Minneapolis). All cell culture reagents were purchased from Gibco/Fisher scientific (Hampton, New Hampshire, USA) unless otherwise specified. Seahorse materials and reagents were purchased from Agilent (California, United States). Cortex.4U, CNS.4U and their culture media were purchased from Axiogenesis (Köln, Germany). Dihydroaeruginoic acid (DHAA, CAS 143209–04-5) was purchased from Santa Cruz Biotechnology, Inc (Dallas, US). Calcein-AM was purchased from Biomol GmbH (Hamburg, Germany).

Ückert A.K., Rütschlin S., Gutbier S., Wörz N.C., Miah M.R., Martins A.C., Hauer I., Holzer A.K., Meyburg B., Mix A.K., Hauck C., Aschner M., Böttcher T, & Leist M. (2023). Identification of the bacterial metabolite aerugine as potential trigger of human dopaminergic neurodegeneration. Environment international, 180, 108229.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!