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Mouse cytokine arrays

Manufactured by R&D Systems
Sourced in United States

Mouse cytokine arrays are multiplex immunoassay panels designed to detect and quantify the expression of various mouse cytokines, chemokines, and other related proteins simultaneously in a single sample. The arrays provide a comprehensive and efficient way to profile the levels of multiple analytes in a single experiment.

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2 protocols using mouse cytokine arrays

1

Cytokine Profiling in AngII/BAPN Murine Aorta

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For the purpose of determining the effects of AngII and BAPN on pro-inflammatory cytokines in the aortic wall, mouse cytokine arrays (R&D Systems, Minneapolis, MN) were performed using isolated protein from mouse aortas at day 4 or 28 according to the manufacturer’s instructions (n=3–5/group at 4 days and n=2/group at 28 days). Protein samples from each group were pooled for analysis, and all samples were run in duplicate.13 (link) Given the high rate of rupture in the AngII/BAPN group at 28 days and the fact that aortic protein samples were pooled, these results were not statistically compared.
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2

Cytokine Profiling of Aortic Wall

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For the purpose of determining the effects of pharmacologic blockade and genetic deletion of androgen receptors and to determine its effect on the pro-inflammatory cytokine mileu of the aortic wall, mouse cytokine arrays (R&D Systems, Minneapolis, MN, USA) were performed using isolated protein from mouse aortas harvested at day 14 after elastase perfusion. Protein samples from each group were pooled for analysis, and all samples were run in duplicate15 (link)–18 (link).
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