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Anti p27 610242

Manufactured by BD

Anti-p27 (610242) is a primary antibody product designed for use in Western blotting and immunohistochemistry applications. The product recognizes the p27 protein, which is a regulator of cell cycle progression. The antibody is produced in mouse and purified using protein A or protein G affinity chromatography.

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2 protocols using anti p27 610242

1

Antibody Characterization for Immunoblotting

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The monoclonal anti-SNX6 antibody 446A was used for immunoblotting studies [37] (link). Other primary antibodies were acquired from the following providers: anti-GST (sc-138), anti-lamin A/C (sc-6215), anti-ERK2 (sc-1647), anti-tubulin (sc-8035), anti-UCP2 (sc-6526), anti-lamin A (sc-20680) anti-SP1 (sc-59-G), and anti-GRP94 (sc-11402) from Santa Cruz Biotechnologies; anti-HA (H-9658) and anti-Flag (F-3165) from Sigma; anti-early endosome antigen 1 (EEA1) (ab14453) from Abcam; anti-GFP (A6455) from Invitrogen; and anti-p27 (610242) from BD Transduction Laboratories. Isotype-specific HRP-coupled secondary antibodies were from Santa Cruz Biotechnology.
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2

Western Blot Analysis of Cell Cycle Regulators

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Samples were loaded on sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) gels. After electrophoresis, gels were transferred to Hybond-C (Amersham Life Sciences) membranes. Then, transferred proteins were subjected to western blotting (WB) using the following antibodies: anti-p21 (sc-6246) and anti-E2F4 (sc-866) from Santa Cruz, anti-p27 (610242) from BD Transduction Laboratories, anti- α-SYN (610787) from BD Biosciences and anti-tubulin (T9026) from Sigma Aldrich.
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