After 4 days of dosing, representative formalin-fixed left and right kidney tissues were immunostained for KIM-1 as described previously.16 (link) Briefly, kidney paraffin blocks were sectioned at 4 μm, mounted on glass slides, deparaffinized, and hydrated in phosphate buffered solution (PBS). Endogenous peroxide was blocked using 3% hydrogen peroxide solution. To avoid nonspecific reaction with primary antibody, slides were pretreated with 10% normal donkey serum. Slides were incubated for 30 min at 4°C with 10% normal rabbit serum before incubation for 2 hours at 4°C with primary rabbit monoclonal antibody to canine KIM-1/TIM-1/HACVR1 (Sino Biological, Inc., 70001-R202) at 1:50. Normal rabbit IgG (Millipore, Billerica, MA, 12 - 370) was used as a negative control. Donkey anti-rabbit IgG Antibody, HRP conjugate, Species Adsorbed (Millipore, AP182P) was used as the secondary antibody at 1:2000 dilution. The immunoreactivities were visualized using VECTASTAIN Elite ABC Systems reagents (Vector Labs, Inc., Burlingame, CA, PK6100) and Liquid DAB + Substrate Chromagen System reagents (DAKO North America, Inc., Carpinteria, CA, K3468) followed by counterstaining with hematoxylin. Slides were scanned at 20× magnification using the NanoZoomer 2.0 HT Digital Pathology system scanner (Hamamatsu Photonics, Bridgewater, NJ).
Nanozoomer 2.0 ht digital pathology system scanner
Manufactured by Hamamatsu Photonics
Sourced in United States
The NanoZoomer 2.0 HT Digital Pathology system scanner is a high-throughput slide scanning device designed for digital pathology applications. It captures high-resolution digital images of tissue samples mounted on microscope slides.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using nanozoomer 2.0 ht digital pathology system scanner
1
Immunostaining of Kidney Tissue for KIM-1
2
Histopathological Evaluation of Kidney Sections
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
At the scheduled necropsy, representative kidneys sections (left and right) were collected, immersed in 10% neutral-buffered formalin solution for up to 48 hr and subsequently transferred to 70% ethanol solution. The preserved tissues were trimmed, processed, embedded in paraffin, sectioned at 4-μm, mounted on glass slides, de-paraffinized, stained with hematoxylin and eosin (H&E), and examined by routine light microscopy. A molecular pathologist conducted an initial assessment of the H&E-stained kidney sections in a blinded-fashion. Kidney lesion characterizations followed the histopathology best practices in the context of non-rodent novel kidney biomarker qualifi cation studies as recommended by the PSTC. The distribution and increased number of cells affected were assigned a qualitative severity grade (e.g., no abnormality noted, minimal, mild, moderate, or severe). Slides were scanned at 20X magnifi cation using the NanoZoomer 2.0 HT Digital Pathology system scanner (Hamamatsu Photonics, Bridgewater, NJ, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!