N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC), N-hydroxy succinimide (NHS), Ethanolamine, Polyoxyethylenesorbitan monolaurate (Tween-20), Phosphate-buffered saline (PBS) (0.01Mphosphate, 0.138 M sodium chloride, 0.0027 M potassium chloride, pH 7.4), Sodium hydroxide (NaOH), hydrochloric acid (HCl), Lysozyme, Ampicillin, Sodium dodecyl sulphate (SDS), N,N-methylene-bisacrylamide, IPTG, skimmed milk powder, 3,3′-diaminobenzidine tetrahydrochloride (DAB), urea, glacial acetic acid, glycine, HRPO and FITC conjugates were purchased from Sigma-Aldrich. All chemicals were of either molecular biology grade or analytical grade and were used as received. All solutions were prepared in Type-II ultrapure water from ELIX Milli-Q system (Millipore).
Elix milli q system
Manufactured by Merck Group
Sourced in United States
The Elix-Milli-Q system is a water purification system designed to produce ultra-pure water for laboratory applications. It combines two stages of purification to remove impurities and provide high-quality water suitable for a wide range of analytical and experimental procedures.
Automatically generated - may contain errors
Lab products found in correlation
Oasis hlb, by Waters Corporation (2 mentions)
Ammonium formate, by Merck Group (2 mentions)
Lc ms grade formic acid, by Merck Group (2 mentions)
Sodium dodecyl sulphate (sds), by Merck Group (1 mentions)
Skimmed milk powder, by Merck Group (1 mentions)
Polyoxyethylenesorbitan monolaurate, by Merck Group (1 mentions)
3 3 diaminobenzidine tetrahydrochloride, by Merck Group (1 mentions)
Isopropyl β d 1 thiogalactopyranoside (iptg), by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Ampicillin, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Glacial acetic acid, by Merck Group (1 mentions)
Urea, by Merck Group (1 mentions)
Ethanolamine, by Merck Group (1 mentions)
Lysozyme, by Merck Group (1 mentions)
Glycine, by Merck Group (1 mentions)
N hydroxysuccinimide, by Merck Group (1 mentions)
N 3 dimethylaminopropyl n ethylcarbodiimide hydrochloride, by Merck Group (1 mentions)
N n methylenebisacrylamide, by Merck Group (1 mentions)
Quartz sand sio2, by Merck Group (1 mentions)
5 protocols using elix milli q system
1
Functionalization and Characterization of Protein Conjugates
2
Quantification of Macrolide Antibiotics
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AZI, ERY-IE, ERY-OX and CLA were kindly supplied by Pliva (Croatia), while ROX was acquired from Sigma-Aldrich (Germany). The remaining eight target compounds, as well as deuterated azithromycin (AZI-d3) and deuterated clarithromycin (CLA-d3), used as internal standards, were purchased from Toronto Research Chemicals (Canada).
HPLC grade methanol (MeOH) and acetonitrile (ACN) were purchased from BDH Prolabo (UK), acetone from Merck (Germany), anhydrous ammonia solution (7 N in MeOH) from Alfa Aesar (Germany), and ACS grade formic acid, as well as quartz sand (SiO2), from Sigma-Aldrich. Ultrapure water was produced by Elix-Milli-Q system (Millipore, USA). Cellulose filters (type D28), used for pressurized liquid extraction (PLE), were produced by Dionex Corporation (USA) and regenerated cellulose filters (0.45 µm), used for the final extract filtration, by Waters (USA). HPLC column ACE C18 PFP (150 × 3 mm; 3 μm) was purchased from Advanced Chromatography Technologies (UK).
HPLC grade methanol (MeOH) and acetonitrile (ACN) were purchased from BDH Prolabo (UK), acetone from Merck (Germany), anhydrous ammonia solution (7 N in MeOH) from Alfa Aesar (Germany), and ACS grade formic acid, as well as quartz sand (SiO2), from Sigma-Aldrich. Ultrapure water was produced by Elix-Milli-Q system (Millipore, USA). Cellulose filters (type D28), used for pressurized liquid extraction (PLE), were produced by Dionex Corporation (USA) and regenerated cellulose filters (0.45 µm), used for the final extract filtration, by Waters (USA). HPLC column ACE C18 PFP (150 × 3 mm; 3 μm) was purchased from Advanced Chromatography Technologies (UK).
3
Quantification of Emerging Pollutants
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Acetaminophen, bezafibrate, bisphenol A, butylparaben, chloramphenicol, clofibric acid, diclofenac, ethylparaben, flufenamic acid, gemfibrozil, ibuprofen, indomethacin, methylparaben, naproxen, propylparaben, salicylic acid, thiamphenicol, triclocarban, triclosan and warfarin from Sigma-Aldrich (The Woodlands,Texas, USA) and tetrahydrocannabinol (THC) and 11-nor-9-carboxy-9-tetrahydrocannabinol (THC-COOH) from LoGiCal (Luckenwalde, Germany) were used as target analytes for QqQ analysis. Calibration standards were prepared by serial dilution of the mixed working solution. Stock and working solutions were stored at −20 °C in the dark [10] (link).
Water used for preparation of calibration standards and LC–MS mobile phase was purified by an Elix Milli-Q system (Millipore, Billerica, MA, USA). Methanol was purchased from Panreac (Castellar del Vallès, Barcelona, Spain) and formic acid was purchased from Amresco (Solon, OH, USA). Ammonium fluoride was acquired from Alfa Aesar GmbH & Co KG (Karlsruhe, Germany).
Water used for preparation of calibration standards and LC–MS mobile phase was purified by an Elix Milli-Q system (Millipore, Billerica, MA, USA). Methanol was purchased from Panreac (Castellar del Vallès, Barcelona, Spain) and formic acid was purchased from Amresco (Solon, OH, USA). Ammonium fluoride was acquired from Alfa Aesar GmbH & Co KG (Karlsruhe, Germany).
4
Analytical Procedures for Biodegradation Compounds
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(Arlesheim, Switzerland), whereas 2-ethyl-5-methyl-3,3-diphenyl-1-pyrroline (EMDP) was obtained from Sigma-Aldrich (Steinheim, Germany). The purity of the reference materials used for confirmatory purposes was ≥ 98%. Chemicals used for growth media were of analytical grade purity while those for molecular analyses were of molecular grade and were supplied by Sigma-Aldrich (USA) and Kemika (Zagreb, Croatia). Difco LB agar was obtained from BD (USA). Ammonium chloride (purity > 99.5%) was purchased from Grammol (Zagreb, Croatia). Formic acid (LC-MS grade) and ammonium formate (purity ≥ 99%) were purchased from Sigma-Aldrich. All other chemicals used for biodegradation media were of analytical grade purity and supplied by Kemika (Zagreb, Croatia). LC-MS grade solvents (acetonitrile and methanol) were products of J.T. Baker (Deventer, the Netherlands).
Ultrapure water was produced using an Elix-Milli-Q system (Millipore, Bedford, MA, USA).
Solid-phase extraction (SPE) cartridges Oasis HLB (60 mg/3 mL) were supplied by Waters (Milford, MA, USA). The individual stock solutions (10 mg/mL and 1 mg/mL) of MTHD were prepared in LC-MS grade methanol.
Ultrapure water was produced using an Elix-Milli-Q system (Millipore, Bedford, MA, USA).
Solid-phase extraction (SPE) cartridges Oasis HLB (60 mg/3 mL) were supplied by Waters (Milford, MA, USA). The individual stock solutions (10 mg/mL and 1 mg/mL) of MTHD were prepared in LC-MS grade methanol.
5
Tramadol Quantification by LC-MS
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TRAM was purchased from Sigma-Aldrich (Steinheim, Germany). N-DM TRAM and deuterated tramadol (TRAM-d3) were purchased from Lipomed (Arlesheim, Switzerland).
The purity of the reference materials used for confirmatory purposes was ≥ 98%. The individual stock solutions (10 mg/mL and 1 mg/mL) of TRAM and N-DM TRAM used for quantitative purposes were prepared in methanol (LC-MS grade). Chemicals used for growth media were of analytical grade purity whereas those for molecular analyses were of molecular grade and were supplied by Sigma-Aldrich (USA) and Kemika (Zagreb, Croatia). Formic acid (LC-MS grade) and ammonium formate (purity ≥ 99%) were purchased from Sigma-Aldrich.
LC-MS grade solvents (acetonitrile and methanol) were products of J.T. Baker (Deventer, the Netherlands). Phenol (purity ≥ 99.5%) and glucose (purity ≥ 99.5 %) were purchased from Sigma-Aldrich. Sulfuric acid (95-97%) was purchased from Merck. Ultrapure water was produced using an Elix-Milli-Q system (Millipore, Bedford, MA, USA).
Solid-phase extraction (SPE) cartridges Oasis HLB (60 mg/3 mL) were supplied by Waters (Milford, MA, USA).
The purity of the reference materials used for confirmatory purposes was ≥ 98%. The individual stock solutions (10 mg/mL and 1 mg/mL) of TRAM and N-DM TRAM used for quantitative purposes were prepared in methanol (LC-MS grade). Chemicals used for growth media were of analytical grade purity whereas those for molecular analyses were of molecular grade and were supplied by Sigma-Aldrich (USA) and Kemika (Zagreb, Croatia). Formic acid (LC-MS grade) and ammonium formate (purity ≥ 99%) were purchased from Sigma-Aldrich.
LC-MS grade solvents (acetonitrile and methanol) were products of J.T. Baker (Deventer, the Netherlands). Phenol (purity ≥ 99.5%) and glucose (purity ≥ 99.5 %) were purchased from Sigma-Aldrich. Sulfuric acid (95-97%) was purchased from Merck. Ultrapure water was produced using an Elix-Milli-Q system (Millipore, Bedford, MA, USA).
Solid-phase extraction (SPE) cartridges Oasis HLB (60 mg/3 mL) were supplied by Waters (Milford, MA, USA).
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