High-purity-grade reagents from Merck (Munich, Germany), Sigma (OOO Sigma-Aldrich Rus, Moscow, Russia), and Helicon (Moscow, Russia) were used. Kits for DNA extraction, restriction, and ligation, oligonucleotides, and Taq polymerase were purchased from Evrogen (Moscow, Russia) and Thermo Fisher Scientific RU (Moscow, Khimki, Russia); kanamycin was produced by Sintez (Moscow, Russia). Yeast extract, bactoagar, tryptone, and peptone were purchased from “Helicon” and “Dia-M” (Moscow, Russia). DNA and protein molecular weight markers were purchased from BioRad (Hercules, CA, USA).
Bactoagar
Manufactured by Helicon
Bactoagar is a solidifying agent used in the preparation of culture media for the growth and isolation of bacteria and other microorganisms. It is a natural polysaccharide derived from red algae. Bactoagar provides a firm, transparent, and nutritious substrate for the cultivation of a wide range of microorganisms.
Automatically generated - may contain errors
2 protocols using bactoagar
1
Molecular Cloning Reagents Sourcing
2
Reagents and Materials for Molecular Biology
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The reagents of chemical grade qualifications were obtained from Merck (Munchen, Germany), Sigma (Sigma-Aldrich Rus LLC, Moscow, Russia), and Helicon (Moscow, Russia). The molecular biology kits for restriction, ligation, Taq polymerase, and oligonucleotides were from Evrogen (Moscow, Russia) and Thermo Fisher Scientific RU (Moscow, Khimki, Russia); kanamycin was from Sintez (Moscow, Russia). Yeast extract, bactoagar, tripton, and pepton were from Helicon and Dia-M (Moscow, Russia). DNA and protein molecular weight markers were from BioRad (California, USA).
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