Hplc grade ethanol

Manufactured by Avantor

Sourced in United States, Germany

HPLC-grade ethanol is a high-purity alcohol solvent used for various analytical and laboratory applications. It is designed to meet the stringent requirements of high-performance liquid chromatography (HPLC) techniques.

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Lab products found in correlation

9 protocols using hplc grade ethanol

Thiacarbocyanine Dyes and Biomacromolecules

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Thiacarbocyanine
(TC) dyes, 3,3′-diethylthiacyanine iodide
([TC0][I]), 3,3′-diethylthiacarbocyanine iodide ([TC1][I]),
and 3,3′-diethylthiadicarbocyanine iodide ([TC2][I]) were purchased
from Sigma Aldrich (St. Louis, MO). Lysozyme (Lys) from chicken egg
white, human transferrin (Trans) (>98%), albumin from human serum
(HSA) (approx. 99%), cytochrome c (Cyt-c), immunoglobulin G from human
serum (IgG), fibrinogen from human plasma (Fib), human hemoglobin
(Hb), ammonium persulfate, sodium phosphate dibasic, and sodium phosphate
monobasic were all also purchased from Sigma Aldrich. Lithium bis(trifluoromethane)sulfonamide
(Li[NTf2]) salt and lithium bis(pentafluoroethanesulfonyl)imide (Li[BETI])
salt were obtained from TCI Portland, Oregon. Artificial urine, HPLC-grade
ethanol, and dichloromethane were acquired from VWR (Batavia, IL).
Triple deionized ultrapure water (18.2 M Ω cm) was obtained
using an Aries high-purity water system (West Berlin, NJ). All reagents
were used as received without further purification.

Pérez R.L., Cong M., Vaughan S.R., Ayala C.E., Galpothdeniya W.I., Mathaga J.K, & Warner I.M. (2020). Protein Discrimination Using a Fluorescence-Based Sensor Array of Thiacarbocyanine-GUMBOS. ACS Sensors, 5(8), 2422-2429.

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Quantitative Analysis of BPA and Analogues

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Bisphenol A (BPA) and BPA analogue standards (>98% purity) were purchased from AccuStandard (New Haven, CT, USA). Labelled BPAA standards (>98% purity) were obtained from Cambridge Isotope Laboratories (Tewksbury, MA, USA). The list of standards and relevant compound information are summarized in Table 1. HPLC-grade ethanol was obtained from VWR International (Mississauga, ON, Canada). HPLC-grade water and LC-MS grade methanol were purchased from EMD Millipore Corporation (Billerica, MA, USA). All standard stock solutions were prepared in LC-MS grade methanol.

Siddique S., Zhang G., Coleman K, & Kubwabo C. (2021). Investigation of the migration of bisphenols from baby bottles and sippy cups. Current Research in Food Science, 4, 619-626.

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Endocrine Disruptor Chemicals Sourcing

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17β-Estradiol (E2, 99% purity), estrone (99%), aldrin (98.6%), dieldrin (99.8%), endosulfan α (I, 99.5%), endosulfan β (II, 99.2%), methoxychlor (99.5%), o,p'-DDT (97.5%), o,p'-DDD (99%), p,p'-DDT (99.1%), p,p'-DDE (99.5%), β-hexachlorocyclohexane (β-HCH, 98.1%), n-butylparaben, n-propylparaben, and bisphenol A (>99%) were purchased from Sigma-Aldrich Company (Dorset, UK). 3-(4-methylbenzylidene)camphor (4-MBC, Eusolex 6300, >99.7%) and octyl-methoxycinnamate (OMC, Eusolex 2292, >98%) were from VWR international (Poole, UK). 3-Benzylidene camphor (3-BC, Unisol-22, >97%) was from Induchem (Volketswil, Switzerland). Genistein was obtained from Alfa Aesar (Lancashire, UK), and 6-acetyl-1,1,2,4,4,7-hexamethyltetraline (AHTN, tonalide) and hexahydrohexamethylcyclopentabenzopyran (HHCB, Galoxolide) from LGC Promochem (Teddington, UK). All chemicals were used as supplied and stock solutions (1 – 10 mM) were prepared in HPLC-grade ethanol (VWR international). Stock solutions and subsequent dilutions were stored at −20°C. All remaining chemicals were purchased from Sigma-Aldrich, unless stated otherwise.

Scholze M., Silva E, & Kortenkamp A. (2014). Extending the Applicability of the Dose Addition Model to the Assessment of Chemical Mixtures of Partial Agonists by Using a Novel Toxic Unit Extrapolation Method. PLoS ONE, 9(2), e88808.

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Licorice Secondary Metabolites Extraction

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Licorice samples (Glycyrrhiza glabra) from the region of Calabria, Italy, were collected in July 2015 and supplied from a local producer. For the extraction of secondary metabolites from this sample, a simple procedure based on solid-liquid extraction assisted by ultrasounds extraction was followed, as described before [15] . The extraction solvent was a binary mixture ethanol/water (1:1, v/v) using a sample-to-solvent ratio 1:5 (w/v) during 60 min. The resulting extract was filtered and evaporated to dryness. Prior injection, the extract was redissolved in water/acetonitrile (3:7, v/v).
HPLC grade ethanol and acetonitrile were purchased from VWR Chemicals (Barcelona, Spain) whereas ultrapure water was produced from a Milli-Q instrument (Millipore, Billerica, MA). Acetic and formic acids were supplied from Sigma-Aldrich (Madrid, Spain), while ammonium acetate was from Panreac (Barcelona, Spain).

Montero L., Ibáñez E., Russo M., Rastrelli L., Cifuentes A, & Herrero M. (2017). Focusing and non-focusing modulation strategies for the improvement of on-line two-dimensional hydrophilic interaction chromatography × reversed phase profiling of complex food samples. Analytica chimica acta, 985.

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Characterization of Plant Metabolites

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Chloroform-d and DMSO-d6 (99.8%) were from Sigma Aldrich Chemie GmbH (Germany).
Methanol-d4 (99.8%), ethanol-d6 (99.8%) and were from Cambridge Isotope Laboratories (USA). D2O (99%) and sodium 3-trimethylsilyl [2,2,3,3-D4]propionate (TSP) were from Goss Scientific Instruments Ltd. (UK). HPLC-grade ethanol 99.7% was obtained from VWR International Ltd. (UK). Hydroxyvalerenic and valerenic acids were from Extrasynthese (France) whilst rutin hydrate from Sigma Chemical Co. (Germany).
Purified water was obtained from a Milli Q gradient system from Millipore (UK).

Prieto J.M., Mellinas-Gomez M, & Zloh M. (2016). Application of diffusion-edited and solvent suppression ¹H-NMR to the direct analysis of markers in valerian-hop liquid herbal products. Phytochemical analysis : PCA, 27(2).

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Topical Tacrolimus Formulation Development and Evaluation

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TCR was purchased from CKD Bio Corporation (Ansan, Korea). Cocoglyceride was obtained from BASF (Ludwigshafen, Germany). The reference product was 0.1% Protopic^® ointment (Astellas Pharm, Tokyo, Japan). Stearic acid was purchased from Tokyo Chemical Industry Co. Ltd (Tokyo, Japan). Poloxamer 188 was supplied by Croda International (Snaith, UK), and soybean lecithin was purchased from Samchun Chemical (Pyeongtaek, Korea). Brij^® 93 and Brij^® 58 were purchased from Sigma Aldrich Corporation (St. Louis, USA). Methylene chloride was purchased from OCI Corporation (Seoul, Korea). High-performance liquid chromatography (HPLC) grade ethanol and acetonitrile were purchased from Honeywell Burdick & Jackson (Muskegon, USA). Water was purified by filtration in the laboratory. HPLC-grade solvents were used for the analysis. Sprague Dawley^® Rats and New Zealand white rabbits were purchased from Samtako Corporation (Osan, Korea).

Kang J.H., Chon J., Kim Y.I., Lee H.J., Oh D.W., Lee H.G., Han C.S., Kim D.W, & Park C.W. (2019). Preparation and evaluation of tacrolimus-loaded thermosensitive solid lipid nanoparticles for improved dermal distribution. International Journal of Nanomedicine, 14, 5381-5396.

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Development of Lidocaine-Based Topical Ointment

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The lidocaine base was a gift from DONG-A Pharm Co., Ltd. (Seoul, Korea). GMS was obtained from Gattefosse Corporation (Lyon, France). Petrolatum was purchased from Wonpoong Pharm Co., Ltd. (Seoul, Korea). Medium-chain triglyceride oil (MCT oil) was obtained from KLK OLEO (Klang, Selangor, Malaysia). Other excipients were supplied by DONG-A Pharm Co., Ltd. (Seoul, Korea). Reference Product A (Ref. A), Prepain^® ointment, was obtained from Ildong Pharm. Co., Ltd. (Seoul, Korea). Water was purified by filtration in the laboratory. High-performance liquid chromatography (HPLC)-grade solvents were used for analysis. HPLC-grade ethanol and acetonitrile were purchased from Honeywell Burdick & Jackson (Muskegon, MI, USA). Sprague Dawley^® rats (SD rats) and Institute of Cancer Research mice (ICR mice) were purchased from Samtako Corporation (Osan, Korea).

Kang J.H., Yoo K.H., Park H.Y., Hyun S.M., Han S.D., Kim D.W, & Park C.W. (2021). Preparation and In Vivo Evaluation of a Lidocaine Self-Nanoemulsifying Ointment with Glycerol Monostearate for Local Delivery. Pharmaceutics, 13(9), 1468.

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Synthesis of Thermoresponsive Polymers

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If not indicated differently, all chemicals were purchased from Sigma Aldrich, Munich, Germany. N-isopropylacrylamide (NIPAM, TCI Germany GmbH, Eschborn, Germany, 97%) and N-isopropylmethacrylamide (NIPMAM, 97%) were recrystallized from n-hexane (VWR International, Darmstadt, Germany, p.a.). N,N′-methylenebisacrylamide (BIS, 99%), ammonium persulfate (APS, 99%), sodium dodecyl sulfate (SDS, 99%), acrylic acid (AAc, 99%) ethanol (HPLC grade, VWR International, Darmstadt, Germany), and polyethyleneimine (PEI, branched 99%) were used as obtained. Water was purified and deionized by an Arium pro VF system (Satorius Stedim Systems GmbH, Göttingen, Germany). N-n-propylacrylamide was synthesized as described by Hirano et al. [50 (link)] via a Schotten–Baumann reaction.

Brändel T., Dirksen M, & Hellweg T. (2019). Tuning the Swelling Properties of Smart Multiresponsive Core-Shell Microgels by Copolymerization. Polymers, 11(8), 1269.

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Synthesis and Characterization of NIPAM and NIPMAM

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N-isopropylacrylamide
(NIPAM) (97% TCI Germany GmbH, Eschborn, Germany) and N-isopropylmethacrylamide (NIPMAM) (97%, Sigma-Aldrich, Munich, Germany)
were recrystallized from n-hexane (p.a., VWR International,
Darmstadt, Germany). N,N′-methylenebisacrylamide
(BIS) (99%, Sigma-Aldrich, Munich, Germany), ammonium persulfate (APS)
(99%, Sigma-Aldrich, Munich, Germany), sodium dodecyl sulfate (SDS)
(99%, Sigma-Aldrich, Munich, Germany), acrylic acid (99%, Sigma-Aldrich,
Munich, Germany), polyethyleneimine (branched, 99%, Sigma-Aldrich,
Munich, Germany), ethanol (HPLC grade, VWR International, Darmstadt,
Germany), silver nitrate (99.999%, Sigma-Aldrich, Munich, Germany),
4-nitrophenole (99%, Carl Roth, Karlsruhe, Germany), and sodium borohydride
(98.0% Sigma-Aldrich, Munich, Germany) were used as obtained. The
synthesis of N-n-propylacrylamide
(NNPAM) was performed via a Schotten–Baumann reaction as described
by Hirano et al.^{53 (link)} Water was purified and
deionized using an Arium pro VF system (Satorius Stedim Systems GmbH,
Göttingen, Germany).

Brändel T., Sabadasch V., Hannappel Y, & Hellweg T. (2019). Improved Smart Microgel Carriers for Catalytic Silver Nanoparticles. ACS Omega, 4(3), 4636-4649.

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