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Cd15 e fluor 450

Manufactured by Thermo Fisher Scientific
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The CD15 e-fluor 450 is a fluorescent-labeled antibody used for cell identification and analysis in flow cytometry applications. It binds to the CD15 antigen, which is expressed on the surface of various cell types, including granulocytes and some monocytes.

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Lab products found in correlation

Facsdiva software, by BD (1 mentions) Cd45 percp, by BD (1 mentions) Cd14 pe cy7, by Thermo Fisher Scientific (1 mentions) Cd24 pe, by Beckman Coulter (1 mentions) Cd64 apc, by Thermo Fisher Scientific (1 mentions) Facscanto 2, by BD (1 mentions) Cd11b pe cy7, by Thermo Fisher Scientific (1 mentions) Cd14 apc, by Thermo Fisher Scientific (1 mentions) Human fc block, by Miltenyi Biotec (1 mentions)

2 protocols using cd15 e fluor 450

1

Immunophenotyping of Cell Populations

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CD59 PE was purchased from Invitrogen, CD235a and CD24PE from Beckman Coulter, CD45PERCP from Becton Dickinson, and CD64APC, CD14PE-Cy7 and CD15 e-fluor 450 from e Bioscience. Fluorescent Aerolysin (FLAER) was obtained from Cedarlane, and lysing reagent (pharmalyse) from Becton Dikinson. For flow studies, acquisition was performed using the FACS Canto II instrument and analysis was performed using the BD FACSDiva™software
Pode-Shakked B., Heimer G., Vilboux T., Marek-Yagel D., Ben-Zeev B., Davids M., Ferreira C.R., Philosoph A.M., Veber A., Pode-Shakked N., Kenet G., Soudack M., Hoffmann C., Vernitsky H., Safaniev M., Lodzki M., Lahad A., Shouval D.S., Levinkopf D., Weiss B., Barg A.A., Daka A., Amariglio N., Malicdan M.C., Gahl W.A, & Anikster Y. (2019). Cerebral and portal vein thrombosis, macrocephaly and atypical absence seizures in Glycosylphosphatidyl Inositol deficiency due to a PIGM promoter mutation. Molecular genetics and metabolism, 128(1-2), 151-161.
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2

Immunophenotyping of MM6 Monocytes

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About 1 × 105 MM6 cells were incubated for 30 min at room temperature with antibodies for CD11b (CD11b-PECy7, eBioscience), CD14 (CD14-APC, eBioscience), CD15 (CD15-eFluor 450, eBioscience), CD33 (CD33-PE P67.6, eBioscience), FVD eFluor® (eBioscience) or with the proper control IgGs. The optimal concentration for each antibody was adjusted according to manufacturer’s instructions. To block unspecific binding of antibodies, human Fc Block (Miltenyi) was added to the cell suspension. After washing with PBS, FACS analysis was performed using FACS Canto II (Becton Dickinson).
Saul M.J., Stein S., Grez M., Jakobsson P.J., Steinhilber D, & Suess B. (2016). UPF1 regulates myeloid cell functions and S100A9 expression by the hnRNP E2/miRNA-328 balance. Scientific Reports, 6, 31995.
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