The antibacterial activity of the processed fibrous membranes was evaluated against known periodontopathogens, i.e. Porphyromonas gingivalis (Pg) (ATCC 33277) and Fusobacterium nucleatum (Fn) (ATCC 25586) using agar diffusion assays (n=3/group/bacteria) [33 (link)]. Bacteria were cultivated in Brain Heart Infusion (BHI) broth supplemented with 5 g yeast extract/L and 5% v/v vitamin K+ hemin (BHI-YE; Becton, Dickinson and Company) at 37°C in an anaerobic GasPak jar for 24 h [33 (link)]. Before testing, the minimum inhibitory concentration (MIC) of ZnO was determined using suspensions of ZnO nanoparticles in PBS at the following concentrations: 100, 250, 500, 1,000, 2,500, 5,000, and 10,000 µg/mL. In brief, 10 µL of each suspension was dropped on cultured blood agar plates containing the bacterial lawns, and the inhibition zones (in mm) were measured after 5 days of incubation [33 (link)]. For the antibacterial activity of the membranes, disk-shaped samples (5-mm in diameter) were prepared and sterilized through ultraviolet (UV) irradiation for 1 h (30 min each side), before placing on the cultured blood agar plates as previously indicated [33 (link), 34 (link)]. The inhibition zone (in mm) of each sample was measured after 5 days of incubation.
Bhi ye
Manufactured by BD
Sourced in United States
BHI-YE is a type of culture media used in microbiology laboratories. It is formulated to support the growth of a wide range of microorganisms, including both aerobic and anaerobic bacteria. The media contains brain heart infusion (BHI) and yeast extract (YE) as the primary nutrient sources.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using bhi ye
1
Antibacterial Activity of Fibrous Membranes
2
Antimicrobial Screening of Fibrous Mats
3
Anaerobic Bacterial Growth Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A brain-heart infusion (BHI) broth supplemented with 5 g yeast extract/L and 5% v/v vitamin K+ hemin (BHI-YE; Becton, Dickinson and Company, Franklin Lakes, NJ, USA) was used to grow the bacteria. Bacterial strains were grown at 37°C in an anaerobic environment, using gas-generating sachets (Gas-Pak EZ; Becton, Dickinson and Company, Franklin Lakes, NJ, USA) to produce the required environment.
4
Cultivating Common Endodontic Pathogens
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Enterococcus faecalis (ATCC 29212) and Porphyromonas gingivalis (ATCC 33277) strains were used in this study. E. faecalis and P. gingivalis were selected as representative common endodontic pathogens that are present in various types of endodontic infections. E. faecalis is a grampositive facultative anaerobe that has been detected in 6777% of cases of secondary root canal infection (18, 19) . On the other hand, P. gingivalis is a gramnegative obligatory anaerobe that has been detected in 4448% of cases of primary root canal infec tion (20, 21) . Each bacterial strain was initially grown on anaerobic blood agar plates (CDC, BioMerieux, Durham, NC, USA), and then grown and maintained as described previously (7, 22, 23) utilizing sterile Brain Heart Infusion broth supplemented with 5 g of yeast extract/L (BHI-YE; Becton Dickinson Co., Franklin Lakes, NJ, USA) containing 5% v/v vitamin K (0.5 mg/mL) and hemin (50 mg/mL) (Remel, Lenexa, KS, USA). Both test bacteria were grown in an anaerobic environment created using gas-generating sachets (Gas-Pak EZ; Becton) and incu bated for 48 h in an incubator at 37ºC. Bacterial growth was confirmed by changes in turbidity at 48 h. The number of colonyforming units/mL for E. faecalis and P. gingivalis after 48 h was 1.78 × 10 8 (optical dentistry = 0.6 at 600 nm) and 3.6 × 10 8 (optical dentistry = 0.67 at 600 nm), respectively.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!