Log In Sign up
The largest database of trusted experimental protocols

Esgro mouse lif medium supplement leukemia inhibitory factor

Manufactured by Merck Group
Visit Supplier

ESGRO mouse LIF Medium Supplement (Leukemia Inhibitory Factor) is a laboratory reagent used to support the growth and maintenance of mouse embryonic stem cells in culture. It contains the leukemia inhibitory factor (LIF), a cytokine that helps maintain the undifferentiated state of mouse embryonic stem cells.

Automatically generated - may contain errors

Lab products found in correlation

Knockout dmem, by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Dmem high glucose, by Merck Group (2 mentions) L glutamine, by Thermo Fisher Scientific (2 mentions) Glutamax, by Thermo Fisher Scientific (2 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (2 mentions) 2 mercaptoethanol, by Thermo Fisher Scientific (2 mentions) 2 mercaptoethanol, by Merck Group (2 mentions) Chir99021, by Selleck Chemicals (2 mentions) Es cell fbs, by Thermo Fisher Scientific (2 mentions) Pd0325901, by Selleck Chemicals (2 mentions)

Spelling variants of this product

Leukemia inhibitory factor (185 citations) ESGRO LIF (69 citations) Leukemia inhibitory factor (LIF; (21 citations) ESGRO Leukemia Inhibitory Factor (10 citations) Mouse leukemia inhibitory factor (9 citations) ESGRO mouse LIF medium supplement (4 citations) ESGRO mouse LIF (4 citations) ESGRO Supplement (3 citations) ESGRO leukemia inhibitory factor LIF (2 citations) ESGRO supplement (LIF, (2 citations)

2 protocols using esgro mouse lif medium supplement leukemia inhibitory factor

1

Feeder-free Culture of Mouse Embryonic Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse embryonic stem cells (ESC) E14 were cultured under feeder-free conditions on 0.1% gelatin coated dishes in Knockout DMEM (Life Technologies) supplemented with 15% FBS (Hyclone), 2 mM L-glutamine (Life Technologies), 0.1 mM non-essential amino acids (Life Technologies), 100 U/ml Penicillin/Streptomycin (Life Technologies), 0.05 mM 2-mercaptoethanol (Sigma), 1,000 U/ml ESGRO mouse LIF Medium Supplement (Leukemia Inhibitory Factor) (Millipore) and maintained at 37ºC with 5% CO2. Cells were fed daily. Primary wild type WT B6 Neo and CRISPR knockout ES cell lines were cultured on irradiated mouse embryonic fibroblast (MEF) feeder layer with high glucose DMEM (Sigma) supplemented with 15% ES Cell FBS (Gibco), 1X non-essential amino acids (Gibco), 1X Glutamax (Thermo Fisher), 100 U/ml Penicillin/Streptomycin (Gibco), 1mM sodium pyruvate (Gibco), 0.1mM 2-mercaptoethanol (Gibco) and further supplemented with PD0325901 and CHIR99021 (Selleckchem). Cells were cultured onto feeder-free 0.1% gelatin coated dishes in Knockout DMEM (Life Technologies) prior to harvest.
Ngan C.Y., Wong C.H., Tjong H., Wang W., Goldfeder R.L., Choi C., He H., Gong L., Lin J., Urban B., Chow J., Li M., Lim J., Philip V., Murray S.A., Wang H, & Wei C.L. (2020). Chromatin Interaction Analyses Elucidate PRC2-bound Silencers’ Roles in Mouse Development. Nature genetics, 52(3), 264-272.
+ Open protocol
+ Expand
2

Feeder-free Culture of Mouse Embryonic Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse embryonic stem cells (ESC) E14 were cultured under feeder-free conditions on 0.1% gelatin coated dishes in Knockout DMEM (Life Technologies) supplemented with 15% FBS (Hyclone), 2 mM L-glutamine (Life Technologies), 0.1 mM non-essential amino acids (Life Technologies), 100 U/ml Penicillin/Streptomycin (Life Technologies), 0.05 mM 2-mercaptoethanol (Sigma), 1,000 U/ml ESGRO mouse LIF Medium Supplement (Leukemia Inhibitory Factor) (Millipore) and maintained at 37ºC with 5% CO2. Cells were fed daily. Primary wild type WT B6 Neo and CRISPR knockout ES cell lines were cultured on irradiated mouse embryonic fibroblast (MEF) feeder layer with high glucose DMEM (Sigma) supplemented with 15% ES Cell FBS (Gibco), 1X non-essential amino acids (Gibco), 1X Glutamax (Thermo Fisher), 100 U/ml Penicillin/Streptomycin (Gibco), 1mM sodium pyruvate (Gibco), 0.1mM 2-mercaptoethanol (Gibco) and further supplemented with PD0325901 and CHIR99021 (Selleckchem). Cells were cultured onto feeder-free 0.1% gelatin coated dishes in Knockout DMEM (Life Technologies) prior to harvest.
Ngan C.Y., Wong C.H., Tjong H., Wang W., Goldfeder R.L., Choi C., He H., Gong L., Lin J., Urban B., Chow J., Li M., Lim J., Philip V., Murray S.A., Wang H, & Wei C.L. (2020). Chromatin Interaction Analyses Elucidate PRC2-bound Silencers’ Roles in Mouse Development. Nature genetics, 52(3), 264-272.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!