All procedures were approved by the Duke University Institutional Animal Care and Use Committee. Mice had free access to food and water and were fed a standard chow (Purina Rodent Chow no. 5001, Purina Mills, St. Louis, MO, USA) prior to being fed a semi-purified 10% fat diet (Research Diets, D12450B) or a 45% fat diet (Research Diets, D03021303) for 12-weeks. The genetic strategy used for generating CrATfl/fl and CrATskm−/− mice was described previously (Seiler et al., 2015 (link)). Glucose tolerance and insulin tolerance tests were performed in the morning 1 hour after food withdrawal. Mice were singly housed with access to water (gel pack). After taking glucose reading using a glucometer at the 0 minute time point, mice were given an intraperitoneal injection of 2.0 g glucose per Kg body or 0.08 U/mouse Humulin, both dissolved in saline. Blood for ELISA insulin measurements was collected at 0, 15, and 60 min. At the time of harvest for proteomics analysis, weights of mice fed the high fat diet averaged 38.6 g (1.1 STE, n=5) for CrATfl/fl and 41.1g (1.8 STE, n=8) for CrATskm−/−. At time of the glucose tolerance test, animals weighed 27.5 g (STE 0.8, n=5) for CrATfl/fl and 37.5g (1.6 STE, n=8) for CrATskm−/−.
Rodent chow no 5001
Manufactured by Purina
Sourced in United States
Purina Rodent Chow no. 5001 is a laboratory animal feed formulated to meet the nutritional requirements of rodents. It provides a complete and balanced diet for the maintenance and growth of rodents in a controlled laboratory setting.
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Lab products found in correlation
4 protocols using rodent chow no 5001
1
High-Fat Diet-Induced Metabolic Phenotypes in CrAT Mice
2
Murine Model for Preclinical Studies
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Male C57BL/6 were purchased from Nanjing Biomedical Research Institute. They were housed in microisolator cages within a barrier facility at 22–24°C on a fixed 12-h light and 12-h dark cycle and were provided ad libitum acidified water and Purina rodent chow No. 5001 (Purina, St Louis, MO). All studies were performed at the vivarium facility of Covance, Greenfield (Indianapolis, USA) or Chem Partner (Shanghai, China) and in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. The protocols for all studies were approved by the Covance Greenfield or Chem Partner Institutional Animal Care and Use Committee.
3
Generation of Skeletal Muscle Crat Knockout Mice
4
Murine Pharmacology and Ovariectomy Studies
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Lexicon mice were generated and studied within the Lexicon vivarium. Mice were group-housed in micro-isolator cages within a barrier facility at 24 °C on a fixed 12-h light and 12-h dark cycle and fed Purina rodent chow No. 5001 (Purina, St. Louis, MO). Diets and acidified water were provided ad libitum. All procedures involving use of live mice were conducted in conformance with Lexicon’s Institutional Animal Care and Use Committee guidelines that were in compliance with state and federal laws and the standards outlined in the Guide for the Care and Use of Laboratory Animals (National Research Council, 1996). Quarterly sentinel surveillance showed no evidence of pathogenic rodent viruses, Mycoplasma, or Helicobacter species in the source colonies. All mice and samples were analyzed after investigator blinding to genotypes and experimental treatments. Male C57BL/6J – 129SvEv F1 hybrid mice were examined in pharmacology studies. The OVX studies examined C57BL/6J mice and Fischer 344 rats. Bilateral ovariectomy (OVX) was performed by standard surgical procedures. Mice and rats were fed either standard mouse chow (Purina) or purified 10% low fat diet (D12450, Research Diets, New Brunswick, NJ). In selected studies NOTUM inhibitor compounds were administered by mixing into the purified diet.
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