Poweropti ecl western blotting detection reagent

Manufactured by Bio-Rad

The PowerOpti-ECL Western blotting detection reagent is a chemiluminescent substrate solution used for the detection of proteins in Western blot analysis. It provides a sensitive and reliable method for visualizing and quantifying target proteins.

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Lab products found in correlation

Protein assay kit, by Bio-Rad (2 mentions) Sqstm1 p62, by Cell Signaling Technology (2 mentions) Immobilon transfer membrane, by Merck Group (2 mentions) Bcl2 associated x (bax), by Santa Cruz Biotechnology (2 mentions) γ tubulin, by Santa Cruz Biotechnology (2 mentions) Cyclophilin a, by Enzo Life Sciences (1 mentions) Trip br1, by Enzo Life Sciences (1 mentions) Hif 1α, by Cell Signaling Technology (1 mentions) β actin, by Santa Cruz Biotechnology (1 mentions) Vdac1, by Santa Cruz Biotechnology (1 mentions) Hsp60, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

ECL reagent (335 citations) ECL detection reagent (75 citations)

2 protocols using poweropti ecl western blotting detection reagent

Protein Expression Analysis by Western Blot

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Cells were centrifuged, washed in ice-cold PBS buffer, and then lysed in RIPA lysis buffer. The amount of protein was quantified using a protein assay kit (Bio-Rad, Korea). Each sample was subjected to SDS-PAGE and transferred to an Immobilon Transfer Membrane (Millipore, Cat#IPVH00010). The filter was incubated with each corresponding antibody, and immunodetection was carried out using the PowerOpti-ECL Western blotting detection reagent (Bio-Rad). The antibodies used in this study were purchased as follows: Bax (Santa Cruz, sc-20067), cyclophilin A (CypA)(Enzo Life Sciences, BML-SA296), HIF-1α (Cell Signaling, Cat#3716S), HMBG1 (Enzo Life Sciences, ALX-210-964), LC3 (Enzo Life Sciences, ALX-803-082), p62/SQSTM1(Cell Signaling, Cat#5114), RIP3 (Santa Cruz, sc-47368), TRIP-Br1 (Enzo Life Sciences, ALX-804-645), XIAP (Cell Signaling, Cat# 2042) and γ-tubulin (Santa Cruz, sc-7396).

Jung S., Li C., Duan J., Lee S., Kim K., Park Y., Yang Y., Kim K.I., Lim J.S., Cheon C.I., Kang Y.S, & Lee M.S. (2015). TRIP-Br1 oncoprotein inhibits autophagy, apoptosis, and necroptosis under nutrient/serum-deprived condition. Oncotarget, 6(30), 29060-29075.

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Western Blot Analysis of Cell Signaling

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Cells were centrifuged, washed in ice-cold phosphate-buffered saline (PBS), and then lysed in radio immunoprecipitation assay (RIPA) lysis buffer. The amount of protein was quantified using a protein assay kit (Bio-Rad, Korea). Each sample was subjected to SDS-PAGE and transferred to an Immobilon Transfer Membrane (Millipore, Cat#IPVH00010). The filter was incubated with each corresponding antibody, and immune-detection was carried out using the PowerOpti-ECL Western blotting detection reagent (Bio-Rad, Korea). The following antibodies were used in this study: PARP (Cell Signaling, Cat#9542S), BAX (Santa Cruz Biotechnology, Cat. sc7480), BAK (Cell signaling, Cat. 3814S), cyclophilin A (CypA)(Enzo Life Sciences, BML-SA296), LC3 (Enzo Life Sciences, ALX-803-082), p62/SQSTM1 (Cell Signaling, Cat#5114), VDAC1 (Santa Cruz Biotechnology, Cat. sc-32063), HSP60 (Santa Cruz Biotechnology, Cat. sc13966), β-actin (Santa Cruz Biotechnology, Cat. sc-47778), and γ-tubulin (Santa Cruz Biotechnology, Cat. sc-7396). The result of Western blot was quantified by using ImageJ program (Image Processing and Analysis in Java, wsr@nih.gov).

Jung S., Moon H.I., Lee B.S., Kim S., Quynh N.T., Yu J., Le D.D., Sandag Z., Lee H., Lee H., Anh N.H., Yang Y., Lim J.S., Kim K.I, & Lee M.S. (2018). Anti-cancerous effect of cis-khellactone from Angelica amurensis through the induction of three programmed cell deaths. Oncotarget, 9(24), 16744-16757.

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