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P38 map kinase antibodies

Manufactured by Cell Signaling Technology
Sourced in United States

P38 MAP kinase antibodies are laboratory reagents used to detect and study the p38 mitogen-activated protein (MAP) kinase, a serine/threonine protein kinase that is involved in various cellular processes, such as inflammation, cell growth, and cell death. These antibodies can be used in various techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to identify and quantify p38 MAP kinase in biological samples.

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4 protocols using p38 map kinase antibodies

1

Collagen-Induced PDGF Signaling Pathway

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Collagen was purchased from Takeda Austria GmbH (Linz, Austria). A PDGF-AB enzyme-linked immunosorbent assay (ELISA) kit was purchased from R&D System, Inc. (Minneapolis, MN, USA). Phospho-specific p38 MAP kinase antibodies and p38 MAP kinase antibodies were obtained from Cell Signaling, Inc. (Beverly, MA, USA). GAPDH antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). All other materials and chemicals were obtained from commercial sources.
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2

PGF2α Signaling Pathway Regulation

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PGF2α was obtained from Sigma-Aldrich Co. (St. Louis, MO, USA). The ELISA kits for mouse IL-6 and mouse VEGF were purchased from R&D Systems, Inc. (Minneapolis, MN, USA). Phospho-specific p44/p42 MAP kinase antibodies, p44/p42 MAP kinase antibodies, phospho-specific p38 MAP kinase antibodies, and p38 MAP kinase antibodies were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). An ECL Western blotting detection system was obtained from GE Healthcare Life Sciences (Buckinghamshire, UK). Negative control-small interfering RNA (siRNA) (Silencer Negative Control #1 siRNA (Neg)) and HSP22-siRNA (s206904 (#1) and s96094 (#2)) were purchased from Ambion (Austin, TX, USA). Other materials and chemicals were obtained from commercial sources.
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3

Investigating Heat Shock Protein 22 Function

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PGF2a was obtained from Sigma-Aldrich Co. (St. Louis, MO, USA). The ELISA kits for mouse IL-6 and mouse VEGF were purchased from R&D Systems, Inc. (Minneapolis, MN, USA). Phospho-specific p44/p42 MAP kinase antibodies, p44/p42 MAP kinase antibodies, phospho-specific p38 MAP kinase antibodies and p38 MAP kinase antibodies were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA). Glyceraldehyde-3phosphate dehydrogenase (GAPDH) antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). An ECL Western blotting detection system was obtained from GE Healthcare Life Sciences (Buckinghamshire, UK). Negative control-small interfering RNA (siRNA) (Silencer Negative Control #1 siRNA (Neg)) and HSP22-siRNA (s206904 (#1) and s96094 (#2)) were purchased from Ambion (Austin, TX, USA).
Other materials and chemicals were obtained from commercial sources.
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4

PGF2-Induced MAPK Signaling Pathway

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PGF2 was obtained from Sigma-Aldrich Co. (St. Louis, MO, USA). The ELISA kits for mouse IL-6 and mouse VEGF were purchased from R&D Systems, Inc. (Minneapolis, MN, USA). Phospho-speci c p44/p42 MAP kinase antibodies, p44/p42 MAP kinase antibodies, phospho-speci c p38 MAP kinase antibodies and p38 MAP kinase antibodies were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA).
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). An ECL Western blotting detection system was obtained from GE Healthcare Life Sciences (Buckinghamshire, UK). Negative control-small interfering RNA (siRNA) (Silencer Negative Control #1 siRNA (Neg)) and HSP22-siRNA (s206904 (#1) and s96094 (#2)) were purchased from Ambion (Austin, TX, USA). Other materials and chemicals were obtained from commercial sources.
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