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Goat anti rat igm alexa fluor 488

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Goat anti–rat IgM Alexa Fluor 488 is a secondary antibody conjugated with the Alexa Fluor 488 fluorescent dye. It is designed to detect and bind to rat IgM antibodies.

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Lab products found in correlation

Lsm 780 confocal microscope, by Zeiss (3 mentions) Streptavidin alexa fluor 555, by Thermo Fisher Scientific (2 mentions) Alexa fluor 555 goat anti rabbit igg, by Thermo Fisher Scientific (2 mentions) Zen black software, by Zeiss (1 mentions) Goat anti rat igm alexa fluor 647, by Thermo Fisher Scientific (1 mentions) Alexa fluor 594 donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Bq123, by Merck Group (1 mentions) Angiotensin 2, by Merck Group (1 mentions) Endothelin 1, by Merck Group (1 mentions) Anti nanog, by Abcam (1 mentions) Anti oct3 4, by Santa Cruz Biotechnology (1 mentions) Nfatc4, by Santa Cruz Biotechnology (1 mentions) Bq788, by Merck Group (1 mentions) Anti ssea 3, by Merck Group (1 mentions) Monoclonal anti α actinin, by Merck Group (1 mentions) Anti anp, by Santa Cruz Biotechnology (1 mentions) Anti cmybp c, by Santa Cruz Biotechnology (1 mentions) Alexa fluor 488 chicken anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Alexa fluor 594 goat anti mouse igg1, by Thermo Fisher Scientific (1 mentions) Goat anti mouse igg2b alexa fluor 594, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Alexa Fluor 488 (6806 citations) Alexa 488 (1863 citations) Alexa Fluor 488 goat anti-rat (92 citations) Alexa Fluor 488 anti-rat (39 citations) Goat anti-rat 488 (13 citations) Alexa Fluor 488 goat anti (9 citations) Alexa 488 anti-rat (8 citations) Alexa Fluor 488 anti (6 citations) Alexa-488 anti-goat (3 citations) Goat anti-IgM Alexa Fluor 488 (2 citations)

4 protocols using goat anti rat igm alexa fluor 488

1

Immunofluorescent Analysis of Thymus Medulla

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Snap-frozen thymus tissues were mounted in OCT, sectioned at 7 µm, and fixed in acetone. The following reagents were used: anti-Aire Alexa Fluor 488 (clone 5H12), anti-Fezf2 (F441, IBL), donkey anti–rabbit IgG Alexa Fluor 594 (Thermo Fisher Scientific), ERTR5 (gift from W. van Ewijk, Leiden University Medical Centre, Leiden, Netherlands), goat anti–rat IgM Alexa Fluor 647 (Thermo Fisher Scientific), goat anti–rat IgM Alexa Fluor 488 (Thermo Fisher Scientific), anti-CD11c Biotin (HL3; BD), anti-CD8 Biotin (53-6.7), and streptavidin Alexa Fluor 555 (Thermo Fisher Scientific). All confocal microscopy was performed on a Zeiss Zen 780 microscope. For quantitation, three to four thymus sections were stained, five images were acquired of medullary and cortical areas, and CD11c+ cells were enumerated. All imaging analysis was conducted using Zeiss Zen Black software.
Cosway E.J., Lucas B., James K.D., Parnell S.M., Carvalho-Gaspar M., White A.J., Tumanov A.V., Jenkinson W.E, & Anderson G. (2017). Redefining thymus medulla specialization for central tolerance. The Journal of Experimental Medicine, 214(11), 3183-3195.
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2

Immunolabeling of Thymic Tissue Sections

Cited 1 time
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Both freshly isolated thymic tissue and thymus grafts were mounted in OCT and snap-frozen in liquid nitrogen before cryosectioning (Cowan et al., 2013 (link)). Antibodies used for immunolabeling of tissue sections were anti-CD4 Alexa Fluor 647 (RM4-5; BioLegend), anti-CD8 biotin (53–6.7, eBioscience), anti-CD31 FITC (PECAM-1; eBioscience), streptavidin Alexa Fluor 555 (Thermo Fisher Scientific), ERTR7 and ERTR5 (van Vliet et al., 1985 (link)), and goat anti-rat IgM Alexa Fluor 488 (Thermo Fisher Scientific). Analysis was performed using a ZEISS LSM 780 confocal microscope and ZEISS Zen Black software.
White A.J., Baik S., Parnell S.M., Holland A.M., Brombacher F., Jenkinson W.E, & Anderson G. (2017). A type 2 cytokine axis for thymus emigration. The Journal of Experimental Medicine, 214(8), 2205-2216.
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3

Immunofluorescence Staining of Pluripotency and Cardiac Markers

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Immunofluorescence staining for pluripotency of iPSCs was performed by using the following primary antibodies: anti‐NANOG (Abcam), anti‐OCT3/4 (Santa Cruz), anti–SSEA 3 (Millipore), anti–SSEA 4 (Millipore), anti–Tra‐1‐60 (Millipore), and anti–Tra‐1‐81 (Millipore). In immunofluorescence staining for cardiac markers, monoclonal anti–α‐actinin (Sigma), monoclonal anti‐cTnT (Thermo Scientific), polyclonal anti‐cTnT (Santa Cruz), monoclonal anti–myosin light chain (MLC)2a (Synaptic Systems), polyclonal anti‐MLC2v (ProteinTech Group), anti‐ANP (Santa Cruz), anti–cMyBP‐C (Santa Cruz), polyclonal anti–cMyBP‐C motif (supplied by C. Witt University of Heidelberg, Heidelberg, Germany), and anti–nuclear factor of activated T cells (NFAT)c4 (Santa Cruz) were used. The isotype‐specific secondary antibodies, Alexa Fluor 488 chicken anti‐rabbit IgG, Alexa Fluor 594 goat anti‐mouse IgG1, Alexa Fluor 488 goat anti‐rat IgM, Alexa Fluor 594 goat anti‐mouse IgM, Alexa Fluor 488 goat anti‐mouse IgG, Alexa Fluor 594 goat anti‐mouse IgG2b, Alexa Fluor 594 chicken anti‐goat IgG, and Alexa Fluor 555 goat anti‐rabbit IgG, were all obtained from Invitrogen. The tested drugs included endothelin‐1 (1.0, 10, 100, or 1000 nmol/L), angiotensin II (100 nmol/L), insulin‐like growth factor 1 (100 nmol/L), phenylephrine (0.05 mmol/L), BQ‐123 (250 nmol/L), and BQ‐788 (100 nmol/L) (all from Sigma).
Tanaka A., Yuasa S., Mearini G., Egashira T., Seki T., Kodaira M., Kusumoto D., Kuroda Y., Okata S., Suzuki T., Inohara T., Arimura T., Makino S., Kimura K., Kimura A., Furukawa T., Carrier L., Node K, & Fukuda K. (2014). Endothelin‐1 Induces Myofibrillar Disarray and Contractile Vector Variability in Hypertrophic Cardiomyopathy–Induced Pluripotent Stem Cell–Derived Cardiomyocytes. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 3(6), e001263.
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4

Immunostaining of Cutaneous Squamous Cell Carcinoma

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Tissue from freshly excised cSCC was obtained from patients during surgery at the Dermatology Department, University Hospital Southampton NHS Foundation Trust. The tissue samples (n=15 tumors) were snap frozen in liquid nitrogen, embedded in OCT medium, cryosectioned and immunostained as described previously (22 (link)). Primary antibodies included anti-CD3 (1:200, DAKO), anti-CD4 (1:50, Abcam), anti-CD8 (1:20, Invitrogen), anti-FOXP3 (1:20, eBioscience), anti-cytokeratin 16 (1:20, Thermo Scientific), anti-cytokeratin 17 (1:20, DAKO), anti CD31 (1:200, eBioscience), anti-CLA (1:200, Biolegend), anti-E-selectin (1:20, R & D Systems) and anti-OX40 (1:200, BD Biosciences). Fluorophore conjugated secondary antibodies comprised Alexa Fluor 488 goat anti-mouse IgG1a, Alexa Fluor 488 goat anti-rat IgM, Alexa Fluor 555 goat anti-rabbit IgG, Alexa Fluor 555 goat anti-rat IgG and Alexa Fluor 633 goat anti-mouse IgG2a (all from Invitrogen). Tissue sections were counterstained with DAPI (Sigma) before being mounted in Mowiol 4–88 (Harco) and imaged using a Zeiss Axioskop 2 fluorescence microscope or a Leica SP5 confocal microscope.
Lai C., August S., Albibas A., Behar R., Cho S.Y., Polak M.E., Theaker J., MacLeod A.S., French R.R., Glennie M.J., Al-Shamkhani A, & Healy E. (2016). OX40+ regulatory T cells in cutaneous squamous cell carcinoma suppress effector T cell responses and associate with metastatic potential. Clinical cancer research : an official journal of the American Association for Cancer Research, 22(16), 4236-4248.
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