Plasma glucose was analyzed following centrifugation by hospital laboratory staff at the shortest possible interval following sample collection using the AU680 Chemistry analyser (Beckman Coulter Inc., High Wycomb, UK) and the hexokinase method. Bloods collected for serum analysis of insulin, lipid and inflammatory markers were centrifuged at 4 °C within an hour of sample collection for 5 min. The separated serum was immediately frozen at −20 °C, with subsequent transfer to a −80 °C freezer. Insulin and c-peptide were quantified by automated immune-assay (Roche Cobas 602; Roche Diagnostics, Basel, Switzerland) with typical CVs < 5%. Total cholesterol, high density lipoprotein cholesterol (HDL-C) and triglycerides were analyzed on a Roche Cobas 702 analyser (Roche Diagnostics). Low density lipoprotein cholesterol (LDL-C) was estimated using the equation of Friedewald et al. [26 (link)]. Maternal C3 was analyzed according to the immunoturbidimetric assay for serum complement C3 (Rx Daytona; Randox Laboratories, Antrim, UK). CRP was analyzed using a biochip array (Evidence Investigator™ Metabolic Syndrome Array II Randox Laboratories, Antrim, UK).
Evidence investigator metabolic syndrome array
Manufactured by Randox
Sourced in United Kingdom
The Evidence Investigator™ Metabolic Syndrome Array is a laboratory equipment product designed for the detection and analysis of various biomarkers associated with metabolic syndrome. It utilizes multiplex technology to simultaneously measure multiple analytes from a single sample, providing a comprehensive assessment of an individual's metabolic profile.
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2 protocols using evidence investigator metabolic syndrome array
1
Comprehensive Metabolic Biomarker Analysis
2
Comprehensive Metabolic Biomarker Analysis
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Plasma glucose was analyzed following centrifugation by hospital laboratory staff at the shortest possible interval following sample collection using the AU680 Chemistry analyser (Beckman Coulter Inc., High Wycomb, UK) and the hexokinase method. Bloods collected for serum analysis of insulin, lipid and inflammatory markers were centrifuged at 4 °C within an hour of sample collection for 5 min. The separated serum was immediately frozen at −20 °C, with subsequent transfer to a −80 °C freezer. Insulin and c-peptide were quantified by automated immune-assay (Roche Cobas 602; Roche Diagnostics, Basel, Switzerland) with typical CVs < 5%. Total cholesterol, high density lipoprotein cholesterol (HDL-C) and triglycerides were analyzed on a Roche Cobas 702 analyser (Roche Diagnostics). Low density lipoprotein cholesterol (LDL-C) was estimated using the equation of Friedewald et al. [26 (link)]. Maternal C3 was analyzed according to the immunoturbidimetric assay for serum complement C3 (Rx Daytona; Randox Laboratories, Antrim, UK). CRP was analyzed using a biochip array (Evidence Investigator™ Metabolic Syndrome Array II Randox Laboratories, Antrim, UK).
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