Ffpe dna mini kit

Manufactured by Qiagen

Sourced in United States

The FFPE DNA Mini Kit is a laboratory equipment product designed for the extraction and purification of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples. The kit provides a method to obtain high-quality DNA from these types of samples.

Automatically generated - may contain errors

Lab products found in correlation

Dna mini kit, by Qiagen (1 mentions) Cpgenome universal methylated dna, by Merck Group (1 mentions) Maxwell tissue dna kit, by Promega (1 mentions) Maxwell instrument, by Promega (1 mentions)

Spelling variants of this product

FFPE DNA Kit (17 citations) Mini Kits (17 citations) DNA kits (7 citations) FFPE Kits (2 citations)

2 protocols using ffpe dna mini kit

Methylation analysis of SFRP2 and SFRP5 in MPM

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Genomic DNA was extracted from MPM cell lines using the DNA Mini Kit (Qiagen, Valencia, CA, USA) and from MPM FFPE samples using the FFPE DNA Mini Kit (Qiagen) according to the manufacturer's instructions. The methylation status of the SFRP2 and SFRP5 promoters in MPM cell lines was determined by MSP or qMSP as described previously [31 (link)]. Primers specific for methylated and unmethylated alleles in MSP were as specified previously [21 (link)]. CpGenome universal methylated DNA (Millipore, Billerica, MA, USA) was used as a positive control for methylation, and normal buffy coat (BC) from a healthy donor was used as a control for the unmethylated locus in each amplification.

Cheng Y.Y., Mok E., Tan S., Leygo C., McLaughlin C., George A.M, & Reid G. (2017). SFRP Tumour Suppressor Genes Are Potential Plasma-Based Epigenetic Biomarkers for Malignant Pleural Mesothelioma. Disease Markers, 2017, 2536187.

+ Open protocol

+ Expand

Comprehensive DNA Extraction and USP8 Sequencing

Check if the same lab product or an alternative is used in the 5 most similar protocols

DNA extraction from the 24 frozen tumours was performed using a Maxwell Instrument and Maxwell Tissue DNA Kit (Promega). The FFPE DNA mini kit (Qiagen) was used for DNA extraction from the 51 FFPE specimens. Exon 14 of USP8 containing the mutational hotspot was amplified by PCR and sequenced as previously described (Perez-Rivas et al. 2015 (link), 2017) . Chromatograms were analysed with the Mutation Surveyor software version v4.09 (Soft Genetics).

Albani A., Perez-Rivas L.G., Tang S., Simon J., Lucia K.E., Colón-Bolea P., Schopohl J., Roeber S., Buchfelder M., Rotermund R., Flitsch J., Thorsteinsdottir J., Herms J., Stalla G., Reincke M, & Theodoropoulou M. (2022). Improved pasireotide response in USP8 mutant corticotroph tumours in vitro. Endocrine-related cancer, 29(8).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!