Human aortic smooth muscle cells (AoSMCs, CC-2571), smooth muscle cell basal medium (SmBM, CC-3181), and SmBM plus Single Quots of supplements (CC-3182) were purchased from Lonza (Walkersville, MD). Recombinant Human TGFβ1 was purchased from Thermo Fisher Scientific (PHG9214). Recombinant Human PDGF-BB (520BB050) was from R&D Systems (Minneapolis, M). Cell Titer-Glo 2.0 Assay kit was purchased from Promega (G9242). Scrambled control and smad2-or MET-specific siRNA were from Thermo Fisher Scientific (Waltham, MA. Scrambled: AM4635; SMAD2:4427037, ID-S8397; MET: 4427038, ID-s8700). Opti-MEM I Reduced Serum Medium, Lipofectamine3000 and Lipofectamine RNAiMAX Transfection Reagent were from Thermo Fisher Scientific (31985062, L3000008 and 13778150). Pierce Fast Western Blot Kit was from Thermo Fisher Scientific (35050). Dead Cell Apoptosis Kit with Annexin V Alexa Fluor 488 & Propidium Iodide (PI) was from Thermo Fisher Scientific (V13241). Nutlin 3a was purchased from Millipore Sigma (SML0580).
Scrambled control and smad2 or met specific sirna
Manufactured by Thermo Fisher Scientific
Scrambled control and smad2-or MET-specific siRNA are laboratory reagents used for gene expression studies. The scrambled control siRNA serves as a negative control, while the smad2-or MET-specific siRNA is designed to target and silence the expression of the smad2 or MET genes, respectively. These reagents are intended for use in cell culture and molecular biology applications.
Automatically generated - may contain errors
Lab products found in correlation
Opti mem 1 reduced serum medium, by Thermo Fisher Scientific (2 mentions)
Recombinant human tgf β1, by Thermo Fisher Scientific (2 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions)
Pierce fast western blot kit, by Thermo Fisher Scientific (2 mentions)
Celltiter glo 2.0 assay kit, by Promega (2 mentions)
Dead cell apoptosis kit with annexin 5 alexa fluor 488 propidium iodide, by Thermo Fisher Scientific (2 mentions)
Nutlin 3a, by Merck Group (2 mentions)
Lipofectamine rnaimax transfection reagent, by Thermo Fisher Scientific (2 mentions)
Smbm plus single quots of supplements cc 3182, by Lonza (2 mentions)
Recombinant human pdgf bb 520bb050, by R&D Systems (2 mentions)
2 protocols using scrambled control and smad2 or met specific sirna
1
Signaling Pathways in Aortic Smooth Muscle Cells
2
Investigating TGFβ1 and PDGF-BB effects on AoSMCs
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