Light microscopy images were obtained using an ADF 1350B microscope equipped with an ADF Live 4h camera (ADF optics Co., Ltd., Jiaxing, China). Cytospins were prepared using a Shandon cytocentrifuge (Thermo Scientific, Langenselbold, Germany). Briefly, 3–4 × 103 cells were spun for 3 min at 1000 rpm. Slides were dried, treated with methanol for 5–10 min, stained with Romanovsky–Giemsa stain (Merck, Burlington, MA, USA) for 15–30 min, dried and analyzed using bright-field microscopy.
Romanovsky giemsa stain
Manufactured by Merck Group
Sourced in United States, Germany
Romanovsky–Giemsa stain is a laboratory staining technique used to differentiate and visualize cellular components in microscopic samples. It is a combination of the Romanowsky and Giemsa stains, which together provide a differential staining of cell structures. The stain is primarily used for the identification and classification of blood cells and parasites in clinical and research settings.
Automatically generated - may contain errors
2 protocols using romanovsky giemsa stain
1
Romanovsky-Giemsa Staining Protocol
2
Cytospin Preparation and Romanovsky-Giemsa Staining
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Light images were obtained using a Zeiss Axioskop 40 microscope equipped with an AxioCamMRc5 camera (Carl Zeiss, Germany). Cytospins were prepared using a Shandon cytocentrifuge (ThermoScientific, Langenselbold, Germany). Briefly, 3–4 × 103 cells were spun for 3 min at 1,000 rpm. Slides were dried, treated with methanol for 5–10 min, stained with Romanovsky-Giemsa stain (Merck, Germany) for 15–30 min, dried and analyzed using bright-field microscopy.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!