Pdgfrα cre c57bl 6 tg pdgfra cre 1clc j mice

All animal procedures were done according to the Association for Research in Vision and Ophthalmology requirements and were approved by the Institutional Animal Care and Use Committee at Wayne State University (A-08-07-15) and conform to NIH guidelines. TLR4 floxed mice (B6(Cg)-Tlr4^tm1.1Karp/J mice) and PDGFRα-Cre (C57BL/6-Tg(Pdgfra-cre)1Clc/J ) mice were purchased from Jackson Laboratories. TLR4 floxed mice were bred with the TLR4-PDGFRα-Cre mice to generate conditional knockout mice in which TLR4 is eliminated in retinal Müller cells. Mice of both sexes were used at 3 months of age. Genotyping and verification of knockout has been previously published (11 (link)).

All animal procedures were done according to the Association for Research in Vision and Ophthalmology requirements and were approved by the Institutional Animal Care and Use Committee of Wayne State University (Protocol 18-08-1575) and conform to NIH guidelines. TLR4 floxed mice (B6(Cg)-Tlr4^tm1.1Karp/J mice) and B6 FVB-Tg (cdh5-cre)7Mlia/J Cre mice were purchased from Jackson Laboratories. After 2 generations, the TLR4 floxed mice were bred with the Cdh5-Cre mice to generate conditional knockout mice where TLR4 is knocked out in vascular endothelial cells (Liu et al., 2017 (link)).
TLR4 floxed mice (B6(Cg)-Tlr4^tm1.1Karp/J mice) and PDGFRα-Cre (C57BL/6-Tg(Pdgfra-cre)1Clc/J mice were purchased from Jackson Laboratories. TLR4 floxed mice were bred to PDGFRα-Cre mice, generating Müller cell specific TLR4 knockout mice. Genotyping and verification of knockout has been previously published (Liu et al., 2017 (link); Liu and Steinle, 2017a (link)).
At 2 months of age, diabetes was induced in the both Cdh5Cre-TLR4 and PDGFRαCre-TLR4 and TLR4 floxed mice using streptozotocin (60mg/kg for 5 consecutive days). Mice were used at 6 months of diabetes for experiments. Both male and female mice were used for all experiments. Table 1 shows body weight and blood glucose levels for the TLR4-endothelial cell KO mice. Table 2 provides the same information for the TLR4-Müller cell KO mice.