Titanium dioxide (TiO2, titania) particles (Titansphere, particle size: 10 μm), Empore SDB-XC and C8 membrane disks were obtained from GL Sciences (Tokyo, Japan). Sequencing-grade modified trypsin was purchased from Promega (Madison, WI, USA). Recombinant protein kinases were obtained from Carna Biosciences (Kobe, Japan). Kinase inhibitors were purchased from Selleck Chemicals (Houston, TX, USA). Water was purified by a Millipore Milli-Q system (Bedford, MA, USA). Alkaline phosphatase from calf intestine, DL-lactic acid, MS-grade Lys-C (lysyl endopeptidase), and all other chemicals were purchased from Fujifilm Wako (Osaka, Japan).
Ms grade lys c
Manufactured by Fujifilm
Sourced in Japan
MS-grade Lys-C is a laboratory protease enzyme used for the digestion of proteins prior to mass spectrometry analysis. It cleaves peptide bonds specifically at the carboxyl side of lysine residues, facilitating the generation of peptide fragments for identification and quantification.
Automatically generated - may contain errors
Lab products found in correlation
Dl lactic acid, by Fujifilm (2 mentions)
Empore sdb xc, by GL Sciences (2 mentions)
Sequencing grade modified trypsin, by Promega (2 mentions)
Kinase inhibitors, by Selleck Chemicals (1 mentions)
Milli q system, by Merck Group (1 mentions)
96 well plate, by Eppendorf (1 mentions)
Ms grade trypsin, by Promega (1 mentions)
Matrix d a r t s tips 20 300 μl, by Thermo Fisher Scientific (1 mentions)
Selumetinib, by Selleck Chemicals (1 mentions)
Inertsep rp c18, by GL Sciences (1 mentions)
Inertsep pls 2, by GL Sciences (1 mentions)
Tc18 sep pak, by Waters Corporation (1 mentions)
3 protocols using ms grade lys c
1
Kinase Assay Protocol with TiO2
2
Mass Spectrometry Sample Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chemicals were purchased from
Sigma-Aldrich (St. Louis, MO) unless specified otherwise. An Eppendorf
96-well plate, matrix D.A.R.T.s tips (20–300 μL), and
automation reservoirs were obtained from Thermo Fisher Scientific
(Hudson, NH). Oasis Mix Mode Anion eXchange (MAX) columns were purchased
from Waters Corporation (Milford, MA). MS-grade trypsin was from Promega
Corporation (Madison, WI). MS-grade LysC was from FUJIFILM Wako Chemicals.
C4 reverse phase resin was purchased from Separation Method Technologies
(Newark, DE) and Fisher Scientific (Waltham, MA). The polyethylene
sheet has a median pore size of 15–45 μm (thickness =
1.57 mm or 0.062 in.; Interstate Specialty Products; Sutton, MA).
Sigma-Aldrich (St. Louis, MO) unless specified otherwise. An Eppendorf
96-well plate, matrix D.A.R.T.s tips (20–300 μL), and
automation reservoirs were obtained from Thermo Fisher Scientific
(Hudson, NH). Oasis Mix Mode Anion eXchange (MAX) columns were purchased
from Waters Corporation (Milford, MA). MS-grade trypsin was from Promega
Corporation (Madison, WI). MS-grade LysC was from FUJIFILM Wako Chemicals.
C4 reverse phase resin was purchased from Separation Method Technologies
(Newark, DE) and Fisher Scientific (Waltham, MA). The polyethylene
sheet has a median pore size of 15–45 μm (thickness =
1.57 mm or 0.062 in.; Interstate Specialty Products; Sutton, MA).
3
Comprehensive Protocol for Multi-Omics Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Titanium dioxide (TiO2, titania) particles (Titansphere, particle size: 10 μm), Empore SDB-XC, C18 and C8 membrane disks, InertSep RP-C18, InertSep PLS-2, and InertSep RP-1 particles were obtained from GL Sciences (Tokyo, Japan). Sep-Pak tC18 and Oasis HLB beads were purchased from Waters (Milford, MA). Sequencing-grade modified trypsin was purchased from Promega (Madison, WI). Selumetinib was purchased from Selleck Chemicals (Houston, TX). MS-grade Lys-C (lysyl endopeptidase), DL-lactic acid, and all other chemicals were purchased from Fujifilm Wako (Osaka, Japan). Water was purified by a Millipore Milli-Q system (Bedford, MA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!