Anti gart

The following antibodies are used in this study: anti-H3K4me1 (Cell Signaling Technology [CST], 5326), anti-H3K4me2 (generated in house), anti-H3K4me3 (CST, 9751), anti-H3K27ac (CST, 8173), H3K27me3 (CST, 9733), anti-MLL3 NT (generated in house), anti-MLL3 MT (generated in house), anti-MLL4 NT (generated in house), anti-MLL4 CT (generated in house), anti-MLL2 (generated in house), anti-SET1A (generated in house), anti-Menin (CST, 6891), anti-MLL1C (CST, 14197), anti-RBBP5 (Bethyl Laboratories, A300-109A), anti-NCOA6 (Bethyl Laboratories, A300-410A), anti-UTX (CST, 33510), anti-PTIP (Bethyl Laboratories, A300-370A), ASH2L (CST, 5019), H3 Ser10-p (CST 53348), CDT1 (CST, 8064), Cyclin B1 (CST, 12231), Geminin (CST, 52508), Cyclin E1 (CST, 20808), Cyclin A2 (CST, 91500), p-cdc2 (CST, 4539), PARP (CST, 9542) Caspase3 (CST, 9662), anti-GART (Santa Cruz Biotechnology, sc-166447), anti-PAICS (Proteintech, 12967-1-AP), Hsp90 (Santa Cruz Biotechnology, sc-13119), and anti-β-tubulin (Developmental Studies Hybridoma Bank, E7). Western blot analysis was performed as previously described (53 (link)).

For western blotting, primary anti-p-AMPK (Thr172, clone 40H9) (1:500, Cat# 2535s), anti-AMPK (1:1000, Cat# 2532s), anti-p-ACC (Ser79, 1:500, Cat# 3661s), anti-ACC (1:1000, Cat# 3662s), anti-PFAS (1:1000, Cat# 61852s) anti-PTEN (1:1000, Cat# 9552S), and anti-PDCD4 (1:1000, Cat# 9535s) antibodies were from Cell Signaling Technology. Primary anti-ATIC (1:1000, clone H-3, Cat# sc-365402), anti-GART (1:1000, clone F-8, Cat# 166447), anti-MTHFD1/1 L (1:1000, clone D-9, Cat# sc-376722), anti-MTHFD2 (1:1000, clone A-2, Cat# sc-390708), anti-AMPD2 (1:1000, clone QQ13, Cat# sc-100504), and anti-NRF2 (1:1000, clone A-10, Cat# sc-365949) antibodies were from Santa Cruz Biotechnology. Primary anti-PPAT (1:1000, Cat# Ab125864) and anti-ADSS/ADSS2 (1:1000, clone EPR12331-52, Cat# Ab174842) antibodies were from Abcam. Primary anti-PAICS (1:1000, clone 2C9, Cat# GTX83950) was from GeneTex. Primary anti-ADSL (1:500, Cat# HPA000525) was from Sigma-Aldrich. Mouse anti-β-actin (1:10,000, clone C4, Santa Cruz, Cat# sc-47778) was used as a loading control. Secondary goat anti-rabbit (1:1000, Cat# 32460) and goat anti-mouse (1:1000, Cat# 32430) antibodies were purchased from Thermo Fisher Scientific.