All chemicals unless mentioned were purchased from Sigma-Aldrich Co. LLC. All the DNA samples (sequence from 5′ to 3′), including DNA1 (GTG TGT GTG TGT GTG TGT GTG TGT GTG TGT ACC TTC CTC CGC), DNA2 (ACT CCC CCA GGT GTG TGT GTG TGT GTG TGT GTG TGT GTG TGT), ATP aptamer (ACC TGG GGG AGT ATT GCG GAG GAA GGT), control aptamer (ATT CTT TTT TAC AAT ACT CCC CCA GGT) and Cy3-labeled DNA2 (Cy3-DNA2, Cy3-ACT CCC CCA GGT GTG TGT GTG TGT GTG TGT GTG TGT GTG TGT), were purchased from Integrated DNA Technologies, Inc. (Coralville, IA, USA). Doxorubicin hydrochloride was purchased from BIOTANG Inc. (Lexington, MA, USA).
Control aptamer
Manufactured by Integrated DNA Technologies
Sourced in United States
Control aptamer is a synthetic, single-stranded DNA or RNA molecule that can bind to specific target molecules. It is designed to serve as a reference or control in assays or experiments involving aptamers.
Automatically generated - may contain errors
Lab products found in correlation
Bovine serum albumin (bsa), by Roche (1 mentions)
Cp004, by R&D Systems (1 mentions)
Hrp conjugated anti dig antibody, by Roche (1 mentions)
Tmb elisa substrate, by Merck Group (1 mentions)
Adenosine triphosphate (atp), by Merck Group (1 mentions)
Mgcl2, by Merck Group (1 mentions)
Pbs buffer, by Merck Group (1 mentions)
3 protocols using control aptamer
1
Fluorescent DNA Aptamer-Doxorubicin Binding Study
2
Quantitative Aptamer-Based Protein Detection
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Streptavidin‐coated 96‐well ELISA plates (R&D systems, CP004) were washed twice in PBS‐T (PBS 1× + 0.05% Tween‐20), then incubated for 1 h at room temperature with 0.025 μM Biotinylated peptides, blocked by incubation with 1% BSA (Sigma, A9647) in PBS for 30 min at 37°C, and then incubated with 0.04 μM digoxigenin (3′‐DIG) AS1411 or control aptamer (Integrated DNA Technologies) in PBS for 1 h at room temperature. Then, plates were incubated with HRP‐conjugated anti‐DIG antibody (Roche, 11207733910) diluted 1/2,000 in 1% BSA‐PBS for 30 min at 37°C. Signal was detected using TMB ELISA substrate (Sigma, T0440). Plates were washed three times before each subsequent step with PBS‐T and five times prior to signal detection. Signal was read using a Tecan5 plate reader reading absorbance at 360 nm wavelength.
3
ATP-specific Aptamer-Nanoparticle Conjugation
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Streptavidin-coated cross-linked iron oxide (CLIO) nanoparticles with hydroxyethyl starch and nominal sizes of 80, 250 and 500 nm were acquired from Micromod Partikeltechnologie GmbH (Rostock, Germany). Biotinylated ATP-specific aptamer (5'-ACC TGG GGG AGT ATT GCG GAG GAA GGT AAA AAA A-3'), DNA linker (5'-CAA TAC TCC CCC AGG TAA AAA AA-3') and control aptamer (5'-ACC TGG GGG AGT ATT AAA AAA AAA AAA AAA AAA A-3') were obtained from Integrated DNA Technologies, Inc. (Coralville, IA). Other chemical reagents including ATP, CTP, GTP, UTP, PBS buffer, MgCl 2 were purchased from Sigma-Aldrich (St. Louis, MO).
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