Log In Sign up
The largest database of trusted experimental protocols

D1 500ng

Manufactured by Abcam
Visit Supplier

D1 500ng is a lab equipment product offered by Abcam. It is a standardized, purified protein sample with a concentration of 500 nanograms per milliliter. The core function of this product is to serve as a reference or control sample for various analytical techniques and assays.

Automatically generated - may contain errors

Lab products found in correlation

Protease inhibitor cocktail, by Abcam (2 mentions) Biotinylated peptide, by GenScript (2 mentions) D1 d2 500ng, by Merck Group (2 mentions) Pierce pull down biotinylated protein protein interaction kit, by Thermo Fisher Scientific (2 mentions)

2 protocols using d1 500ng

1

Affinity Purification of PTPσ Interactors

Check if the same lab product or an alternative is used in the 5 most similar protocols
For pulldown experiments, we used the Pierce Pull-Down Biotinylated Protein:Protein Interaction Kit (Thermo Scientific 21115). 100µg/ml of biotinylated-peptide (Genscript) was incubated overnight on an orbital shaker at 4°C with streptavidin beads. Following incubation, extra biotin was added and allowed to incubate overnight to ensure the binding of all streptavidin. After three washes with TBS, either recombinant GST tagged-PTPσ ICD (D1/D2-500ng, Sigma, D1 500ng, Abcam), spinal cord lysate from either wild type or PTPσ null mice, or brain lysate from an adult female Sprague Dawley rat. Neural tissue was quickly extracted and flash frozen with liquid nitrogen. The tissue was homogenized in Tissue Homogenization Buffer (20mM Tris, 0.5mM EDTA, 0.5mM EGTA and 8% Sucrose, pH 7.4) and 1:500 Protease inhibitor cocktail (Abcam) on ice. The lysate was centrifuged at 13,000 rpm for 20 min before addition to the beads. 150µl of each lysate was added to the beads and allowed to incubate overnight at 4°C. Following three washes, beads were incubated with elution buffer for 10 minutes at room temperature. Beads were then centrifuged at 12,000 rpm to collect eluted lysate.
Lang B., Cregg J., DePaul M., Tran A., Xu K., Dyck S., Madalena K., Brown B., Weng Y., Li S., Karimi-Abdolrezaee S., Busch S., Shen Y, & Silver J. (2014). Modulation of the proteoglycan receptor PTPσ promotes recovery after spinal cord injury. Nature, 518(7539), 404-408.
+ Open protocol
+ Expand
2

Affinity Purification of PTPσ Interactors

Check if the same lab product or an alternative is used in the 5 most similar protocols
For pulldown experiments, we used the Pierce Pull-Down Biotinylated Protein:Protein Interaction Kit (Thermo Scientific 21115). 100µg/ml of biotinylated-peptide (Genscript) was incubated overnight on an orbital shaker at 4°C with streptavidin beads. Following incubation, extra biotin was added and allowed to incubate overnight to ensure the binding of all streptavidin. After three washes with TBS, either recombinant GST tagged-PTPσ ICD (D1/D2-500ng, Sigma, D1 500ng, Abcam), spinal cord lysate from either wild type or PTPσ null mice, or brain lysate from an adult female Sprague Dawley rat. Neural tissue was quickly extracted and flash frozen with liquid nitrogen. The tissue was homogenized in Tissue Homogenization Buffer (20mM Tris, 0.5mM EDTA, 0.5mM EGTA and 8% Sucrose, pH 7.4) and 1:500 Protease inhibitor cocktail (Abcam) on ice. The lysate was centrifuged at 13,000 rpm for 20 min before addition to the beads. 150µl of each lysate was added to the beads and allowed to incubate overnight at 4°C. Following three washes, beads were incubated with elution buffer for 10 minutes at room temperature. Beads were then centrifuged at 12,000 rpm to collect eluted lysate.
Lang B., Cregg J., DePaul M., Tran A., Xu K., Dyck S., Madalena K., Brown B., Weng Y., Li S., Karimi-Abdolrezaee S., Busch S., Shen Y, & Silver J. (2014). Modulation of the proteoglycan receptor PTPσ promotes recovery after spinal cord injury. Nature, 518(7539), 404-408.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!