Eos kiss x7i camera

The SDS-PAGE gel image shown in Fig. 3 was taken using a Limited-Stage II gel imager (AMZ System Science, Osaka, Japan), equipped with an EOS Kiss X7i camera (Canon, Tokyo, Japan) with an EF-S18-55 mm lens, and a standard white-light box, on which the gel was placed. Aperture, shutter speed, and ISO were set to F20, 1/6 s, and 100, respectively. No image processing was performed.

The cells were seeded in 24-well plates at a density of 2 × 10⁴ cells/well for B16-F1 and 5 × 10⁴ cells/well for HEMn-DP. After incubation for 24 h, cells were treated with each agent for 72 h. At the end of the treatment, B16-F1 cells were observed under a Nikon TS-1000 microscope (Nikon, Tokyo, Japan) and photographed using a Canon EOS Kiss X7i camera (Canon, Tokyo, Japan). To measure melanin content, the medium was removed, and the cells were dissolved in 120 μL of 1 M NaOH at 80 °C for 20 min. One hundred microliters of the solution were transferred to a 96-well plate, and the absorbance at 415 nm was measured using a microplate reader (iMark, BioRad, Tokyo, Japan). Melanin content was calculated from the absorbance ratio relative to that of the control culture.