1 2 dimyristoyl rac glycero 3 methoxypolyethylene glycol 2000 mpeg dmg

Ovalbumin (OVA), cholesterol and distearoyl phosphatidylcholine (DSPC) were acquired from Sigma-Aldrich, Inc. (St. Louis, MO). Lipidoid (C12-200) was purchased from Xinjiahecheng Medical Chemistry Corporation (Hubei, China). 1,2-Dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000 (mPEG-DMG) was purchased from NOF Corporation (Tokyo, Japan). The Giemsa staining kit for BALF was purchased from Baso Biotechnology Corporation (Guangdong, China).
Antibodies against F4/80 and CD206 were purchased from Santa Cruz Biotechnology (CA, USA). Antibodies against MBD2 and Arginase-1 were purchased from Abcam (MA, USA). The anti-GAPDH antibody was purchased from Proteintech (Hubei, China), while anti-Ym1 antibody was ordered from Thermo Fisher Scientific (PA, USA). PE-conjugated anti-human CD14, PE-conjugated anti-mouse F4/80, and APC-conjugated anti-mouse CD11c antibodies, ELISA kits for IL-4 and TNF-α were purchased from BioLegend (CA, USA).

Recombinant human TGF-β1 (Cat: 100-21) was purchased from the PeproTech Corporation (Cranbury, NJ, USA). Antibodies against E-cadherin (Cat: Sc-7870), N-cadherin (Cat: Sc-8424), Vimentin (sc-58899) and GAPDH(Sc-47724) were obtained from the Santa Cruz Biotechnology (Dallas, TX, USA). The crystal violet staining solution (Cat: C0121) and the ChIP assay kit (Cat: P2078) were purchased from the Beyotime Biotechnology (Shanghai, China). The lipidoid C12-200 was purchased from the Xinjiahecheng Medical Chemistry Corporation (Wuhan, Hubei, China). 1,2-Dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000 (mPEG-DMG) was ordered from the NOF Corporation (Tokyo, Japan). The CCK-8 assay kit (Cat: 40203ES60) was originated from Yeasen Biotechnology (Wuhan, China), and the Lipofectamine 3000 (Cat: L3000015) was obtained from the Invitrogen Corporation (Shanghai, China). All other reagents were obtained from Sigma (St. Louis, MO, USA).