Fzr1 cdh1

Mouse monoclonal antibodies against HA (sc-7392, 1:1000), Flag (Anti-DDDDK-tag, M185-3L, 1:1000) (MBL Life Science, Woburn, MA, USA), Histone H3 (CST-9751, 1:1000), GAPDH (sc-32233, 1:1000), ubiquitin (sc-8017, 1:1000) and normal mouse IgG (sc-2025, 1:1000) were purchased from Santa Cruz Biotech, Dallas, TX, USA; Histone H3 (CST-9751, 1:1000) were purchased from Cell Signaling Technology, Danvers, MA USA, rabbit polyclonal antibodies against FAH (LS-C165918, 1:1000) and FZR1/Cdh1 (Cat no. #34-2000, Invitrogen, 1:1000) were purchased from LS-Bio, Seattle, WA, USA and Invitrogen, Carlsbad, CA, USA, respectively. In addition, 488/594-conjugated secondary antibodies (Cat no. #A21207, Cat no. #A21203, 1:200) (Life Technologies, Carlsbad, CA, USA) were used. Protein A/G Plus Agarose beads (sc-2003, Santa Cruz Biotech, Dallas, TX, USA); protease inhibitor cocktail (Cat no. #B14012, Bimake.com, Houston, TX, USA), protein translation inhibitor cycloheximide (CHX; Cat no. #239765, Merck, Kenilworth, NJ, USA), RIPA buffer (Cat no. #R2002, Bioseong), Protein 5X sample buffer (Cat no. #EBA-1052, ELPIS BIOTECH, Taejon, Korea), proteasomal inhibitor MG132 (Cat no. #S2619, Selleckchem, Houston, TX, USA) and TUBE 2 (Cat no. #UM402, Life Sensors, Malvern, PA, USA) were also used.

Mouse monoclonal antibodies against PAH (sc-271258, 1:50), Flag (Anti-DDDDK-tag, M185-3L, 1:1000) (MBL Life Science, Woburn, MA, USA), ubiquitin (sc-8017, 1:1000), HA (sc-7392, 1:1000), GAPDH (sc-32233, 1:1000), and normal mouse IgG (sc-2025, 1:1000) were purchased from Santa Cruz Biotech, Dallas, TX, USA. PAH (MAB5278, 1:1000, Merck Millipore, Kenilworth, N.J., USA), rabbit polyclonal antibodies against FZR1/Cdh1 (Cat no. #34-2000, 1:1000 Invitrogen, Carlsbad, CA, USA), and 488/594-conjugated secondary antibodies (Cat no. #A21207, Cat no. #A21203, 1:200) (Life Technologies, Carlsbad, CA, USA) were also used. In addition, we used Protein A/G Plus Agarose beads (sc-2003, Santa Cruz Biotech, Dallas, TX, USA), proteasomal inhibitor MG132 (Cat no. #S2619, Selleckchem, Houston, TX, USA), protein translation inhibitor cycloheximide (CHX; Cat no. #239765, Merck, Kenilworth, NJ, USA), and protease inhibitor cocktail (Cat no. #B14012, Bimake.com, Korea).