Cells were resuspended in 2% FBS/phosphate-buffered saline (PBS), and cell surface staining was performed using fluorescein isothiocyanate-conjugated mouse anti-human CD105, fluorescein isothiocyanate-conjugated mouse anti-human CD90, fluorescein isothiocyanate-conjugated mouse anti-human CD146, phycoerythrin-conjugated mouse anti-human CD29, phycoerythrin-conjugated mouse anti-human CD34 (BD Pharmingen, USA), or phycoerythrin-conjugated mouse anti-human stromal precursor antigen-1 (STRO-1; Invitrogen, USA) antibodies for 30 min at 4 °C. Isotype-identical antibodies served as controls. The cells were analysed using a Beckman Coulter EPICS XL flow cytometer.
Phycoerythrin conjugated mouse anti human cd34
Manufactured by BD
Sourced in United States
Phycoerythrin-conjugated mouse anti-human CD34 is a fluorescently labeled antibody that binds to the CD34 antigen on the surface of human cells. It can be used for the identification and enumeration of CD34-positive cells in flow cytometry applications.
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2 protocols using phycoerythrin conjugated mouse anti human cd34
1
Phenotypic Characterization of Stem Cells
2
Characterizing hWJ-MSC Surface Markers
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hWJ-MSCs at passage 3 were harvested and stained with the following antibodies: phycoerythrin-conjugated mouse anti-human CD34, CD73, CD105, and HLA-DR; allophycocyanin-conjugated mouse anti-human CD79a and CD90 (BD Pharmingen, San Diego, CA, USA). The isotype-matched immunoglobulins IgG1-phycoerythrin and IgG1-allophycocyanin were used as negative controls under the same conditions. All steps were performed according to the manufacturer's instructions. The profiles of hWJ-MSCs were analyzed by flow cytometry (FACSCalibur, Becton, Dickinson and Company, Franklin Lakes, NJ, USA).
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