Sigma’s Caspase-3 Colorimetric Assay Kit (CASP-3-C, Sigma, St. Louis, MO, USA) was used to measure the activity of active caspase-3 in a manner dependent on the hydrolysis of the peptide substrate (Ac-DEVD-pNA) by caspase-3, which releases the p-nitroaniline (pNA) moiety. The concentration of pNA released was calculated from a calibration curve prepared with defined pNA solutions as described by Nichoson et al. [33 (link)]. The degree of caspase-3 activation was determined by calculating the multiples of the optical density (OD) for the treated and control groups.
Sigma s caspase 3 colorimetric assay kit casp 3 c
Manufactured by Merck Group
Sourced in United States
The Sigma's Caspase-3 Colorimetric Assay Kit (CASP-3-C) is a laboratory product designed to detect and quantify the activity of the Caspase-3 enzyme. Caspase-3 is a key effector of apoptosis, a form of programmed cell death. The kit utilizes a colorimetric substrate to measure Caspase-3 activity in cell and tissue lysates.
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2 protocols using sigma s caspase 3 colorimetric assay kit casp 3 c
1
Colorimetric Assay for Caspase-3 Activity
2
Caspase-3 Activation in Melanoma
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The effect of crude extract and F1 fraction on the activation of caspase-3 on the mitochondria from melanoma and extra tumoral cells were evaluated according to the instructions of the Sigma’s caspase-3 colorimetric assay kit (CASP-3-C; Sigma-Aldrich, Saint Louis, USA). Eventually, the concentration of the p-nitroaniline (pNA) released from the substrate at 405 nm was calculated for assessment caspase-3 activity or from a standard curve prepared with defined p-nitroaniline solutions (Seydi et al., 2016b).
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