Application suite las v4

For the woody bamboos, mature leaf blades were taken from the branches at the mid-culm, whereas for the herbaceous bamboos mature leaves were taken from the third node from the base. Fresh plant material was fixed in FAA 50 (Johansen, 1940) and later stored in 70% ethanol. Found on leaves of Arthrostylidiinae species, the green stripe was excluded from this work due to its anatomical peculiarities in relation to the remainder of the leaf blade (Judziewicz et al., 1999) .
Samples from the middle portion of the leaf blade were embedded in polyethylene glycol 1500 solution (adapted from Richter, 1985) (link) and cross-sectioned with a rotary microtome. Sections were cleared in sodium hypochlorite 50%, washed in distilled water, stained with Astra blue and Safranin (Bukatsh, 1972) , and finally mounted on semi-permanent slides with glycerol. Also, a maceration technique was performed by the Jeffrey's method (Johansen, 1940) in order to describe the epidermal features.
Descriptions were primarily based on Ellis (1976 (link)Ellis ( , 1979)) (link), and optical images were obtained on a Leica DM4000B microscope using the software Leica Application Suite LASV4.0.