The antibodies that were used in this study were PhosphoJAK2 (Tyr1007, Tyr1008), JAK1 antibody (EPR349[N]) (ab133666), and Phospho-JAK1 (phospho-Y1022 + Y1023) antibody (EPR1899[2 (link)]) (ab138005) (Abcam, Cambridge, UK); Phospho-Jak2 [p Tyr1007, p Tyr1008] antibody (SY24-03), Phospho-STAT1 [p Tyr701] antibody, STAT3 (232209) antibody, and Phospho-STAT3 (Tyr705) antibody (1004G) (Novus Bio., Cambridge, UK); SOCS7 antibody (PA5-44102), JAK2 monoclonal antibody (691R5), and actin monoclonal antibody (ACTN05 [C4]) (Thermo Fisher Scientific). Secondary horseradish peroxidase (HRP)-linked anti-mouse and anti-rabbit IgG antibodies were supplied in the Western Breeze™ Chemiluminescent Kit (Thermo Fisher Scientific).
Actin monoclonal antibody actn05 c4
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom, China
The Actin monoclonal antibody (ACTN05 [C4]) is a laboratory reagent used for the detection and analysis of actin, a key structural protein found in eukaryotic cells. This antibody is designed to specifically bind to and identify actin, facilitating its visualization and quantification in various experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
Western breeze chemiluminescent kit, by Thermo Fisher Scientific (1 mentions)
Phospho jak2, by Abcam (1 mentions)
Phospho jak1, by Abcam (1 mentions)
Ab70374, by Abcam (1 mentions)
Biotin, by Thermo Fisher Scientific (1 mentions)
Cav 1 sirna, by GenePharma (1 mentions)
Rictor sirna, by GenePharma (1 mentions)
Phospho akt ser473, by Cell Signaling Technology (1 mentions)
Sgc 7901, by iCell Bioscience (1 mentions)
2 protocols using actin monoclonal antibody actn05 c4
1
Antibodies for JAK/STAT Signaling Analysis
2
Rictor and Cav1 Regulation in Gastric Cancer
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Primary antibodies used were rabbit monoclonal anti-Rictor (ab70374, Abcam, Cambridge, MA), anti-Cav 1(Rabbit mAb #3276), Akt (pan) (Rabbit mAb #4691, Phospho-Akt (Ser473) (Rabbit mAb #4060) (Cell Signaling Technology, Shanghai, China), Caspase-3 monoclonal antibody (CPP32-4-1-18), Bcl-2 polyclonal antibody (PA5-11379), Bax monoclonal antibody (6A7), actin monoclonal antibody (ACTN05 (C4) and Biotin (ThermoFisher, Shanghai, China). Rictor plasmid (Addgene #1860) and Cav 1 plasmid (Addgene27703) were purchased from Addgene (USA). Rictor-siRNA and Cav 1-siRNA were purchased from GenePharma Co., Ltd. (Shanghai, China). Ly294002 (#9901) was purchased from Cell Signaling Technology. Human GC cell lines SGC-7901 and AGS were purchased from iCell Bioscience Inc (Shanghai, China) in Feb 2018.
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