The short hairpin RNA (shRNA) specifically targeting LSINCT5 (called sh-LSINCT5) was synthesized by GenePharma (Shanghai, China), and nonspecific shRNA was used as negative control (sh-NC). The full-length sequence of LSINCT5 was inserted into pcDNA3.1 vector (pcDNA-LSINCT5, Invitrogen, Grand Island, NY, USA) and an empty pcDNA vector as negative control. The miR-30a mimic, miR-30a inhibitor, and corresponding NC were synthesized by RiboBio (Guangzhou, China). According to the manufacturer’s protocol, MCF-7 cells was stably transfected with all plasmids for 48 hours using Lipofectamine 3000 reagent (Invitrogen, Grand Island, NY, USA).
Mir 30a mimic
Manufactured by RiboBio
Sourced in China
The MiR-30a mimic is a synthetic RNA molecule designed to mimic the function of the endogenous microRNA miR-30a. MicroRNAs are small, non-coding RNA molecules that play a role in gene expression regulation. The MiR-30a mimic can be used in research applications to study the biological functions of miR-30a.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mir 30a mimic
1
Modulating LSINCT5 Expression in MCF-7 Cells
2
Transfection of miR-30a and TET1 in A549 cells
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A549 cell lines were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Cell culture was prepared as previously described [37 (link),38 (link)]. miR-30a mimic and inhibitor and TET1 siRNA were synthesized by RiboBio Co., Ltd. (Guangzhou, China). A total of 1 × 105 cells was seeded in 24-well plates and cultivated with 1640 medium containing 10% newborn calf serum for 24 h. An amount of 1.25 µL of 20 µM miR-30a mimic/inhibitor or 1.25 µL of 20 µM TET1 siRNA was diluted with 50 µL 1× riboFECTTM CP (RiboBio Co., Ltd., Guangzhou, China)buffer and incubated for 5 min at room temperature. A total of 5 µL riboFECTTM CP reagents was added and incubated for 15 min at room temperature. The mixed liquid was added to 443.75 µL 1640 medium without 10% newborn calf serum. Cells were incubated with the mixed liquid for 48 h.
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