Without further purification, the collected N-glycans were directly permethylated according to the solid-phase permethylation procedure(7 (link)). The matrix solution was prepared by dissolving 10 mg of 2,5-dihydroxybenzoic acid in a volume of 1 mL of 50% methanol containing 1 mM sodium acetate. Glycans were spotted directly onto a stainless steel MALDI plate and mixed with an equal volume of matrix solution (0.5 – 1 μL). MALDI-MS was carried out on an MDS SCIEX 4800 (Applied Biosystems, Carlsbad, CA) using the interactive mode. The external calibration was performed using the ProteoMass Peptide MALDI-MS calibration kit (Sigma-Aldrich, St. Louis, MO). MS data were processed using Data Explorer 4.9 (Applied Biosystems, Carlsbad, CA).
Mds sciex 4800
Manufactured by Thermo Fisher Scientific
The MDS SCIEX 4800 is a mass spectrometry instrument designed for analytical applications. It utilizes advanced technology to provide accurate and reliable measurements of chemical compounds. The core function of the MDS SCIEX 4800 is to perform mass spectrometry analysis.
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Lab products found in correlation
2 protocols using mds sciex 4800
1
Permethylation and MALDI-MS Analysis of N-Glycans
2
MALDI-MS Analysis of Glycans
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MALDI-MS was carried
out on a MDS SCIEX 4800 (Applied Biosystems, Carlsbad, CA) equipped
with an Nd:YAG laser with 355 nm wavelength of <500 ps pulse and
200 Hz repetition rate. The spectrometer was operated in the positive
ion reflectron mode. External calibration was performed using the
ProteoMass Peptide MALDI-MS calibration kit (Sigma-Aldrich, St. Louis,
MO). The matrix solution was prepared by dissolving 10 mg of DHB in
a volume of 1 mL of 50% acetonitrile containing 8 mM sodium acetate.
Glycans were spotted directly onto a stainless steel MALDI plate and
mixed with an equal volume of matrix solution (0.5–1 μL)
until air-dry. The MS data were initially processed and exported using
Data Explorer 4.9 (Applied Biosystems, Carlsbad, CA).
out on a MDS SCIEX 4800 (Applied Biosystems, Carlsbad, CA) equipped
with an Nd:YAG laser with 355 nm wavelength of <500 ps pulse and
200 Hz repetition rate. The spectrometer was operated in the positive
ion reflectron mode. External calibration was performed using the
ProteoMass Peptide MALDI-MS calibration kit (Sigma-Aldrich, St. Louis,
MO). The matrix solution was prepared by dissolving 10 mg of DHB in
a volume of 1 mL of 50% acetonitrile containing 8 mM sodium acetate.
Glycans were spotted directly onto a stainless steel MALDI plate and
mixed with an equal volume of matrix solution (0.5–1 μL)
until air-dry. The MS data were initially processed and exported using
Data Explorer 4.9 (Applied Biosystems, Carlsbad, CA).
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