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Control rigg1

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Control rIgG1 is a recombinant immunoglobulin G1 (rIgG1) antibody that serves as a control reagent in various immunological experiments and assays. Its core function is to provide a baseline comparison for experimental samples.

Automatically generated - may contain errors

Lab products found in correlation

Rmil 7, by BioLegend (2 mentions) Rmil 2, by BioLegend (2 mentions) Anti il 13, by Thermo Fisher Scientific (2 mentions) Soluble anti cd28, by BioXCell (2 mentions) Cell stimulation cocktail, by Thermo Fisher Scientific (2 mentions) Anti il 4, by BioLegend (2 mentions) Celltrace violet cell proliferation kit, by Thermo Fisher Scientific (2 mentions) Anti cd3ε, by BioLegend (2 mentions) Rmil 25, by Johnson & Johnson (2 mentions)

2 protocols using control rigg1

1

ILC2-Mediated Modulation of Tumor M-MDSCs

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Intestinal ILC2s were sorted and cultured in complete RPMI (RPMI-1640 + 10% FCS + penicillin/streptomycin + 2-mercaptoethanol) with 10 ng/mL rm-IL-2 (Biolegend, 575406), 10 ng/mL rm-IL-7 (Biolegend, 577806), and 20 ng/mL rm-IL-25 (Janssen) for 3 days at 37°C, and supernatant collected (ILC2-SNT). ILC2-SNT were incubated with anti-IL-4 (Biolegend, 504122) and anti-IL-13 (eBioscience, 16-7135-85) neutralizing antibodies (αIL-4/13Ab-ILC2-SNT), or control rIgG1 (Biolegend, 400432 and eBioscience, 16-4301-85) antibodies (conAb-ILC2-SNT) for 1 hour on ice. Tumor M-MDSCs were sorted and incubated with αIL-4/13Ab-ILC2-SNT or conAb-ILC2-SNT for 2 hours at 37°C. Subsequently, sorted splenic CD8+ T cells (Live CD45+ TCRγδ-CD4-CD8+) stained with the CellTrace Violet Cell Proliferation Kit (ThermoFisher, C34557), were added to the ILC2-SNT-M-MDSC culture, and stimulated for 3 days with plate-coated anti-CD3ε (Biolegend, 100360) and 2 μg/mL soluble anti-CD28 (Bio X Cell, BE0015-1) at 37°C. Cell Stimulation Cocktail (eBioscience, 00-4975-93) was added for the last 4 hours of culture, before antibody staining. For Arginase 1 expression analysis in ILC2-SNT-treated M-MDSCs, tumor or splenic M-MDSCs were sorted and cultured with αIL-4/13Ab-ILC2-SNT or conAb-ILC2-SNT for 2 days, and stained with antibodies for flow cytometry.
Jou E., Rodriguez-Rodriguez N., Ferreira A.C., Jolin H.E., Clark P.A., Sawmynaden K., Ko M., Murphy J.E., Mannion J., Ward C., Matthews D.J., Buczacki S.J, & McKenzie A.N. (2022). An innate IL-25-ILC2-MDSC axis creates a cancer-permissive microenvironment for Apc-mutation-driven intestinal tumorigenesis. Science immunology, 7(72), eabn0175.
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2

ILC2-Mediated Modulation of Tumor M-MDSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Intestinal ILC2s were sorted and cultured in complete RPMI (RPMI-1640 + 10% FCS + penicillin/streptomycin + 2-mercaptoethanol) with 10 ng/mL rm-IL-2 (Biolegend, 575406), 10 ng/mL rm-IL-7 (Biolegend, 577806), and 20 ng/mL rm-IL-25 (Janssen) for 3 days at 37°C, and supernatant collected (ILC2-SNT). ILC2-SNT were incubated with anti-IL-4 (Biolegend, 504122) and anti-IL-13 (eBioscience, 16-7135-85) neutralizing antibodies (αIL-4/13Ab-ILC2-SNT), or control rIgG1 (Biolegend, 400432 and eBioscience, 16-4301-85) antibodies (conAb-ILC2-SNT) for 1 hour on ice. Tumor M-MDSCs were sorted and incubated with αIL-4/13Ab-ILC2-SNT or conAb-ILC2-SNT for 2 hours at 37°C. Subsequently, sorted splenic CD8+ T cells (Live CD45+ TCRγδ-CD4-CD8+) stained with the CellTrace Violet Cell Proliferation Kit (ThermoFisher, C34557), were added to the ILC2-SNT-M-MDSC culture, and stimulated for 3 days with plate-coated anti-CD3ε (Biolegend, 100360) and 2 μg/mL soluble anti-CD28 (Bio X Cell, BE0015-1) at 37°C. Cell Stimulation Cocktail (eBioscience, 00-4975-93) was added for the last 4 hours of culture, before antibody staining. For Arginase 1 expression analysis in ILC2-SNT-treated M-MDSCs, tumor or splenic M-MDSCs were sorted and cultured with αIL-4/13Ab-ILC2-SNT or conAb-ILC2-SNT for 2 days, and stained with antibodies for flow cytometry.
Jou E., Rodriguez-Rodriguez N., Ferreira A.C., Jolin H.E., Clark P.A., Sawmynaden K., Ko M., Murphy J.E., Mannion J., Ward C., Matthews D.J., Buczacki S.J, & McKenzie A.N. (2022). An innate IL-25-ILC2-MDSC axis creates a cancer-permissive microenvironment for Apc-mutation-driven intestinal tumorigenesis. Science immunology, 7(72), eabn0175.
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