We used the following chemicals: ATP disodium salt (Sigma-Aldrich, St. Louis, MO, United States), PPADS (20 μM, Abcam, USA, a non-selective P2 purinergic receptor antagonist), NF449 (1 μM, Cayman, USA, P2X1R antagonist), AF-353 (0.1 μM, donated by China Pharmaceutical University, P2X3R antagonist), 5-BDBD (10 μM, Sigma-Aldrich, United States, P2X4R antagonist), AZ10606120 (1 μM, Tocris Bioscience, USA, P2X7R antagonist), BzATP (30 μM, Alomone Labs, Israel, P2X7R agonist), recombinant mouse SCF protein (10 ng/mL, R&D Systems, USA), penicillin and streptomycin (100 μg/mL; Gibco, USA), fibronectin (30 μg/mL; Sigma-Aldrich, United States), Fluo 4-AM (Solarbio, China, calcium indicator), Histamine ELISA Kit (Yifeixue, China), IL-1β ELISA Kit (Yifeixue, China), Trizol (Vazyme Biotech, China), HiScript II Q RT SuperMix for qPCR (Vazyme Biotech, China), Taq MasterMix (Vazyme Biotech, China), AceQ qPCR SYBR Green Master Mix (Vazyme Biotech, China), ATP Content Assay Kit (Yifeixue, China), salicylic acid (Yuanye Biotech, China), aspirin (Yuanye Biotech, China), Rabbit Anti-P2RX7 antibody (Bioss, China)
Bzatp
Manufactured by Alomone
Sourced in United States, Israel
BzATP is a laboratory reagent that functions as a potent P2X7 receptor agonist. It is commonly used in experimental settings to activate and study the P2X7 receptor and its associated signaling pathways.
Automatically generated - may contain errors
Lab products found in correlation
Salicylic acid, by Yuanye Bio-Technology (1 mentions)
Penicillin and streptomycin, by Thermo Fisher Scientific (1 mentions)
Nf449, by Cayman Chemical (1 mentions)
Aspirin, by Yuanye Bio-Technology (1 mentions)
Fibronectin, by Merck Group (1 mentions)
Atp disodium salt, by Merck Group (1 mentions)
Az10606120, by Bio-Techne (1 mentions)
Recombinant mouse scf protein, by R&D Systems (1 mentions)
Fluo 4 am, by Solarbio (1 mentions)
Ppads, by Abcam (1 mentions)
5 bdbd, by Merck Group (1 mentions)
Hiscript 2 q rt supermix for qpcr, by Vazyme (1 mentions)
Trizol, by Vazyme (1 mentions)
Aceq qpcr sybr green master mix, by Vazyme (1 mentions)
Taq master mix, by Vazyme (1 mentions)
2 protocols using bzatp
1
Investigating Purinergic Signaling in Mast Cell Activation
2
Pharmacological Compound Delivery in Cells
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Stock solutions of BzATP (Alomone Labs, Jerusalem, Israel) and AFC-5128 (Affectis Pharmaceuticals AG, Dortmund, Germany) were diluted and applied in HEPES-buffered extracellular solution. The drug solutions were delivered to the recorded cells by a valve-controlled fast multibarrel superfusion system with a common outlet approximately 350 µm in diameter (Automate Scientific, California, USA). The application tip was routinely positioned approximately 1 mm away from and ∼50 µm above the surface of the recorded cells. A computer connected to Digidata 1550B controlled the onset and duration of each drug application. The time course of the drug application was calibrated using fluorescein dye.
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