1,2-dimyristoleoyl-sn-glycero-3-phosphocholine (DMoPC), egg yolk sphingomyelin (SM), and cholesterol were purchased from Avanti Polar Lipids (Alabaster, AL, USA). 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) labeled with Atto 633, sodium chloride (NaCl), and chloroform (HPLC grade) were purchased from Merck KGaA (Darmstadt, Germany). 4-(2-hydroxyethyl)piperazine-1-ethanesulphonic acid (HEPES PUFFERAN®) was obtained from Carl Roth GmbH&Co KG (Karlsruhe, Germany). Calcium chloride (CaCl2) was purchased from P.P.H. STANLAB sp. j., Lublin, Poland. Optical adhesive glue Norland 68 was purchased from Thorlabs Sweden AB (Mölndal, Sweden). All the materials were used without further purification. The ultrapure water (ddH2O) was obtained by using Milli-Q reference water purification system from Merck KGaA (Darmstadt, Germany).
Hepes pufferan
Manufactured by Carl Roth
Sourced in Germany, France
HEPES Pufferan is a zwitterionic organic chemical compound used as a biological buffer to maintain a stable pH in aqueous solutions. It is commonly used in cell culture, biochemical, and molecular biology applications.
Automatically generated - may contain errors
Lab products found in correlation
Millipore integral 5 apparatus, by Merck Group (2 mentions)
Hplc grade chloroform, by Merck Group (1 mentions)
Milli q reference water purification system, by Merck Group (1 mentions)
Cholesterol, by Avanti Polar Lipids (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Hplc grade, by Merck Group (1 mentions)
Glycine, by Carl Roth (1 mentions)
Chloroform, by Avantor (1 mentions)
Creatinine, by Merck Group (1 mentions)
Sodium phosphate, by Merck Group (1 mentions)
Kolliphor p188, by Merck Group (1 mentions)
Kolliphor p 407, by Merck Group (1 mentions)
Ethylenediaminetetraacetic acid, by Merck Group (1 mentions)
Trisodium citrate, by Merck Group (1 mentions)
N hydroxysuccinimide, by Merck Group (1 mentions)
Ethyl acetate, by Carl Roth (1 mentions)
Resomer rg503h, by Boehringer Ingelheim (1 mentions)
4 protocols using hepes pufferan
1
Lipid Bilayer Formation Protocol
2
Silicone Elastomer Preparation Protocol
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Kits for the preparation of silicone elastomers (MED-4735; NuSil Technology, Carpinteria, USA); dexamethasone (polymorphic Form A; Discovery Fine Chemicals, Dorset, UK); calcium chloride dihydrate, magnesium sulfate tetrahydrate, potassium chloride, sodium chloride and 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES) (HEPES Pufferan; Carl Roth, Lauterbourg, France); acetonitrile (HPLC grade; Fisher Scientific, Illkirch, France); MilliQ water (obtained with a Millipore Integral 5 apparatus; Millipore Corporation, Billerica, USA).
3
Silicone Elastomer Preparation and Dexamethasone Milling
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Kits for the preparation of silicone elastomers (MED-4735; NuSil Technology, Carpinteria, USA); dexamethasone and dexamethasone sodium phosphate (dexamethasone phosphate) (Discovery Fine Chemicals, Dorset, UK); calcium chloride dihydrate, magnesium sulfate tetrahydrate, potassium chloride, sodium chloride and 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES) (HEPES Pufferan; Carl Roth, Lauterbourg, France); acetonitrile (HPLC grade; Fisher Scientific, Illkirch, France); MilliQ water (obtained with a Millipore Integral 5 apparatus; Millipore Corporation, Billerica, USA).
The dexamethasone sodium phosphate powder was used as received, or (if indicated) milled as follows: 1 g drug was milled in a stainless-steel jar with a stainless-steel ball for 3 min at 30 Hz (Retsch MM400; Retsch, Haan, Germany).
The dexamethasone sodium phosphate powder was used as received, or (if indicated) milled as follows: 1 g drug was milled in a stainless-steel jar with a stainless-steel ball for 3 min at 30 Hz (Retsch MM400; Retsch, Haan, Germany).
4
Polymeric Nanoparticle Fabrication and Characterization
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PLGA (Resomer RG 503 H,
poly(d ,l -lactide-co-glycolide))
was obtained from Boehringer Ingelheim (Ingelheim, Germany), and PLGA
Resomer Sample CR Type RG 50:50 Mn2300 (PLGA 2300) was kindly provided
by Evonik Nutrition & Care GmbH (Darmstadt, Germany). Trimethoprim,
Kolliphor P 188 (Pluronic F68), Kolliphor P 407 (Pluronic F127), N-hydroxy succinimide (NHS), ethylenediaminetetraacetic
acid (EDTA), creatinine, trisodium citrate, and sodium phosphate were
purchased from Sigma-Aldrich (St. Louis, MO, USA). Fluorescein-labeled
wheat germ agglutinin (f-WGA) was provided by Vector Laboratories,
Inc. (Burlingame, CA, USA). Chloroform (≥98%) was obtained
from VWR International (Pennsylvania, USA), and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
(EDAC), ethyl acetate (≥99.5%), sodium chloride, disodium oxalate,
HEPES (Pufferan, ≥99.5% buffer grade), and glycine were purchased
from Carl-Roth GmbH & Co.KG (Karlsruhe, Germany).
poly(
was obtained from Boehringer Ingelheim (Ingelheim, Germany), and PLGA
Resomer Sample CR Type RG 50:50 Mn2300 (PLGA 2300) was kindly provided
by Evonik Nutrition & Care GmbH (Darmstadt, Germany). Trimethoprim,
Kolliphor P 188 (Pluronic F68), Kolliphor P 407 (Pluronic F127), N-hydroxy succinimide (NHS), ethylenediaminetetraacetic
acid (EDTA), creatinine, trisodium citrate, and sodium phosphate were
purchased from Sigma-Aldrich (St. Louis, MO, USA). Fluorescein-labeled
wheat germ agglutinin (f-WGA) was provided by Vector Laboratories,
Inc. (Burlingame, CA, USA). Chloroform (≥98%) was obtained
from VWR International (Pennsylvania, USA), and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
(EDAC), ethyl acetate (≥99.5%), sodium chloride, disodium oxalate,
HEPES (Pufferan, ≥99.5% buffer grade), and glycine were purchased
from Carl-Roth GmbH & Co.KG (Karlsruhe, Germany).
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