Mouse monoclonal anti α tubulin t5168

Immunoblotting was performed as previously reported^{16 (link),17 (link)}. The antibodies used were mouse monoclonal anti-VDAC1 (ab14734), mouse monoclonal anti-SDHA (ab14715), mouse monoclonal anti-Rieske (ab14746), mouse monoclonal anti-SIRT1 (ab110304), rabbit polyclonal anti-histone H3 (ab1791) from Abcam (Cambridge, UK); mouse monoclonal anti-HSP60 (SPA-806) from Enzo Life Sciences (Farmingdale, NY); guinea pig polyclonal anti-p62 (GP62-C) from Progen (Heidelberg, Germany); rabbit monoclonal anti-LC3A/B (#12741), rabbit monoclonal anti-phospho-Ser37/46-4EBP1 (#2855), rabbit polyclonal anti-4EBP1 (#9452), rabbit polyclonal anti-phospho-ser235/236 S6 (#2211), rabbit monoclonal anti-S6 (#2217), rabbit polyclonal anti-phospho-Ser473 Akt (#9271), rabbit polyclonal anti-Akt (#9272), rabbit monoclonal anti-FoxO3a (#2497), Lamin A/C (#2032) from Cell Signaling Technologies (Danvers, MA, USA); mouse monoclonal anti-GAPDH (G8795), mouse monoclonal anti-α-tubulin (T5168) from Sigma Aldrich (St. Louis, MO, USA); and rabbit polyclonal anti-acetylated histone H3 (382158) from Millipore (Billerica, MA, USA).

N-phosphonacetyl-l-aspartate (PALA, NSC224131) was obtained from the National Cancer Institute (NCI)/Division of Cancer Treatment and Diagnosis (DCTD)/Developmental Therapeutics Program (DTP) Open Chemical Repository (http:dtp.cancer.gov). Aquaphor® and Neosporin® were purchased from Target (Cleveland Heights, OH). Gentamycin solution was purchased from Sigma (St. Louis, MO). Rabbit monoclonal anti-CAD antibody (EP711Y) was purchased from Novus Biologicals (Littleton, CO). Rabbit polyclonal anti-RICK (H-300) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Rabbit monoclonal anti-GAPDH (14C10) was purchased from Cell Signaling Technologies (Danvers, MA). Mouse monoclonal anti-α-tubulin (T5168) was purchased from Sigma. Antibodies against LL-37 (sc-166770) and HBD2 (sc-20798) were purchased from Santa Cruz Biotechnology. Anti-HBD3 antibody was purchased from Novus Biologicals (NB200-117). The plasmids, pcDNA3-HA-NOD1, pcDNA3-HA-NOD2, pBVIII-Luc, and pCMV-βgal were gifts of Gabriel Nuñez (University of Michigan) and have been previously described^{56 (link)}. MISSION short hairpin RNA (shRNA) constructs targeting CAD (NM_004341.3-6910s21c1), NOD2 (NM_022162.1-2959s1c1), RIP2 (NM_003821.5-2364s21c1), and non-targeting shRNA control (SHC002) were purchased from Sigma.