780 zen microscope

Manufactured by Zeiss

The Zeiss 780 Zen microscope is a high-performance confocal laser scanning microscope designed for advanced imaging applications. It features a modular design and integrates a range of imaging modes, including fluorescence, transmitted light, and differential interference contrast (DIC) imaging. The microscope is equipped with multiple laser lines, enabling researchers to visualize a variety of fluorescent probes and samples. The Zeiss 780 Zen microscope is a versatile tool for a wide range of applications, including cell biology, neuroscience, and materials science.

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Lab products found in correlation

Prolong gold, by Thermo Fisher Scientific (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Gata3, by Jackson ImmunoResearch (1 mentions) Gata3, by Thermo Fisher Scientific (1 mentions) Fjk 16, by Thermo Fisher Scientific (1 mentions) Af488 donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Cd3 clone 145 2c11, by Thermo Fisher Scientific (1 mentions)

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Zeiss 780 Zen confocal fluorescence microscope

2 protocols using 780 zen microscope

Immunofluorescence Analysis of Mouse Lung Tissue

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Lungs were infused with 50% O.C.T. (Tissue-Tek) in PBS immediately after mice were killed. Lungs were subsequently frozen in 100% O.C.T. compound, followed by sectioning. 6 μm-thick sections of frozen lung tissue were cut, fixed in cold acetone at 4 °C for 20 min and then stored at −20 °C before staining. Antibodies raised against the following mouse antigens were used: CD3 (biotinylated, clone eBio500A2, Thermo Fisher), FoxP3 (APC conjugated, clone FJK-16 s, Thermo Fisher), GATA-3 (unconjugated, clone TWAJ, Thermo Fisher), IL-7Rα (efluor660 conjugated, clone A7R34, Thermo Fisher). Detection of GATA-3 expression required amplification of the signal: the primary antibodies were detected with donkey anti-rat-IgG-FITC (Jackson ImmunoResearch), then rabbit anti-FITC-AF488 (Life Technologies) and then with donkey anti-rabbit-IgG-AF488 (Life Technologies). Biotinylated anti-CD3 antibodies were detected with SA-AF555 (Life Technologies). Sections were counterstained with DAPI (Invitrogen) and mounted using ProLong Gold (Invitrogen). Slides were analyzed on a Zeiss 780 Zen microscope (Zeiss).

Halim T.Y., Rana B.M., Walker J.A., Kerscher B., Knolle M.D., Jolin H.E., Serrao E.M., Haim-Vilmovsky L., Teichmann S.A., Rodewald H.R., Botto M., Vyse T.J., Fallon P.G., Li Z., Withers D.R, & McKenzie A.N. (2018). Tissue-Restricted Adaptive Type 2 Immunity Is Orchestrated by Expression of the Costimulatory Molecule OX40L on Group 2 Innate Lymphoid Cells. Immunity, 48(6), 1195-1207.e6.

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Immunofluorescent Staining of Mouse Tissue

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Tissue sections from experimental mice were cut and stained as described previously (Mackley et al., 2015 (link)). Briefly, 6-μm-thick sections of tissue were cut, fixed in formalin at 4 °C for 20 min and then stored at −20 °C before staining. Antibodies raised against the following mouse antigens were used: CD3 (clone 145–2C11, dilution 1:100, eBioscience) IL-7Rα (clone A7R34, dilution 1:25, Thermo Scientific), purified RORγt (clone AFKJS-9, dilution 1:30, Thermo Scientific) and HLA-DR (clone M5/114.15.2, dilution 1:200, Biolegend). Detection of RORγt expression required amplification of the signal as described previously (Mackley et al., 2015 (link)). Purified rat primary antibodies against the transcription factors were detected with donkey anti-rat-IgG-FITC (dilution 1:100, molecular probes), and then rabbit anti-FITC-AF488 (dilution 1:100, molecular probes) or with donkey anti-rabbit-IgG-AF488 (dilution 1:100, molecular probes). Biotinylated anti-CD3 antibodies were detected with SA-AF555 (dilution 1:100, molecular probes). Sections were counterstained with DAPI (Invitrogen) and mounted using ProLong Gold (Invitrogen). Slides were analyzed on a Zeiss 780 Zen microscope (Zeiss).

Goc J., Lv M., Bessman N.J., Flamar A.L., Sahota S., Suzuki H., Teng F., Putzel G.G., Eberl G., Withers D.R., Arthur J.C., Shah M.A, & Sonnenberg G.F. (2021). Dysregulation of ILC3s unleashes progression and immunotherapy resistance in colon cancer. Cell, 184(19), 5015-5030.e16.

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