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Rabbit anti mouse gfap

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Rabbit anti-mouse GFAP is a polyclonal antibody produced in rabbits and targeted against the mouse glial fibrillary acidic protein (GFAP). GFAP is a type III intermediate filament protein that is commonly used as a marker for astrocytes in the central nervous system.

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Lab products found in correlation

Clone m1 70, by Thermo Fisher Scientific (2 mentions) Clone soia15, by Thermo Fisher Scientific (2 mentions) Rat anti mouse mbp, by Merck Group (2 mentions) Goat anti rabbit af488, by Thermo Fisher Scientific (2 mentions) Live dead viability cytotoxicity kit for mammalian cells, by Thermo Fisher Scientific (2 mentions) Rat anti mouse ki67, by Thermo Fisher Scientific (2 mentions) Clone 12, by Merck Group (2 mentions) Rat anti mouse cd11b, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

Anti-GFAP (49 citations) Mouse anti-GFAP (19 citations) Rabbit anti-GFAP (14 citations)

2 protocols using rabbit anti mouse gfap

1

Immunofluorescence Staining of Cultured Cells

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Cell cultures were fixed in 4% paraformaldehyde (pH 7.4) for 15-20 min at RT and blocked in 10% normal goat serum with 0.1% Triton-X-100 for 1 hr. Cells were then incubated with primary antibody overnight at 4°C and in secondary antibodies for 1 hr at RT. Cells were counterstained with DAPI for 5 min at RT. Primary antibodies used were rabbit anti-mouse Olig2 (1:200; cat. no. AB9610, Millipore), rat anti-mouse MBP (1:200; clone 12, Millipore), rat anti-mouse Ki67 (1:200; clone SoIA15, eBioscience), rabbit anti-mouse GFAP (1:200; cat. no. Z0334, Dako) and rat anti-mouse CD11b (1:200; clone M1/70, eBioscience) and secondary antibodies used were goat anti-rabbit AF488 (1:1000; cat. no. A-11008) and goat anti-rat AF594 (1:1000; cat. no. A-11007; both Life Technologies). For live/dead staining unfixed cells were stained using the LIVE/DEAD® Viability/Cytotoxicity Kit for mammalian cells (Life Technologies). Immunofluorescence was detected using an EVOS microscope at 10X magnification, and n = 6 wells per condition, with one mean value from multiple images calculated for each well.
Dombrowski Y., O’Hagan T., Dittmer M., Penalva R., Mayoral S.R., Bankhead P., Fleville S., Eleftheriadis G., Zhao C., Naughton M., Hassan R., Moffat J., Falconer J., Boyd A., Hamilton P., Allen I.V., Kissenpfennig A., Moynagh P.N., Evergren E., Perbal B., Williams A.C., Ingram R.J., Chan J.R., Franklin R.J, & Fitzgerald D.C. (2017). Regulatory T cells promote myelin regeneration in the Central Nervous System. Nature neuroscience, 20(5), 674-680.
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2

Immunofluorescence Staining of Cultured Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell cultures were fixed in 4% paraformaldehyde (pH 7.4) for 15-20 min at RT and blocked in 10% normal goat serum with 0.1% Triton-X-100 for 1 hr. Cells were then incubated with primary antibody overnight at 4°C and in secondary antibodies for 1 hr at RT. Cells were counterstained with DAPI for 5 min at RT. Primary antibodies used were rabbit anti-mouse Olig2 (1:200; cat. no. AB9610, Millipore), rat anti-mouse MBP (1:200; clone 12, Millipore), rat anti-mouse Ki67 (1:200; clone SoIA15, eBioscience), rabbit anti-mouse GFAP (1:200; cat. no. Z0334, Dako) and rat anti-mouse CD11b (1:200; clone M1/70, eBioscience) and secondary antibodies used were goat anti-rabbit AF488 (1:1000; cat. no. A-11008) and goat anti-rat AF594 (1:1000; cat. no. A-11007; both Life Technologies). For live/dead staining unfixed cells were stained using the LIVE/DEAD® Viability/Cytotoxicity Kit for mammalian cells (Life Technologies). Immunofluorescence was detected using an EVOS microscope at 10X magnification, and n = 6 wells per condition, with one mean value from multiple images calculated for each well.
Dombrowski Y., O’Hagan T., Dittmer M., Penalva R., Mayoral S.R., Bankhead P., Fleville S., Eleftheriadis G., Zhao C., Naughton M., Hassan R., Moffat J., Falconer J., Boyd A., Hamilton P., Allen I.V., Kissenpfennig A., Moynagh P.N., Evergren E., Perbal B., Williams A.C., Ingram R.J., Chan J.R., Franklin R.J, & Fitzgerald D.C. (2017). Regulatory T cells promote myelin regeneration in the Central Nervous System. Nature neuroscience, 20(5), 674-680.
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