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Snail ha

Manufactured by Addgene
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The Snail-HA is a lab equipment product that serves as an epitope tag for protein expression and detection. It is a small, genetically encoded tag derived from the protein Snail, which can be fused to a target protein to enable its identification and purification using antibodies specific to the HA epitope.

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Lab products found in correlation

Pepmute sirna transfection reagent, by SignaGen (2 mentions) 8xgtiic, by Addgene (2 mentions) Human snail or slug sirnas, by Santa Cruz Biotechnology (2 mentions) Flag snail, by Addgene (2 mentions) Slug myc, by Addgene (2 mentions) Flag taz, by Addgene (2 mentions) Flag yap, by Addgene (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)

2 protocols using snail ha

1

Silencing TEAD and SNAIL/SLUG genes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human or mouse TEF-1, TEF-3, TEF-4 and TEF-5 (TEAD1–4) siRNAs, and human SNAIL or SLUG siRNAs were obtained from Santa Cruz Technology. To silence gene expression, PepMute siRNA Transfection Reagent (SignaGen Laboratories) was used for all siRNAs transfection. Flag-Snail, Snail-HA, Slug-Myc, Flag-YAP, Flag-TAZ, and 8xGTIIC were obtained from Addgene. HA-Runx2 and 6xOSE reporter constructs were provided by G. Stein 16 (link). Snail, Slug, YAP and TAZ mutants were constructed by PCR. Where indicated, Flag-YAP and Flag-TAZ were cloned into pLentilox-IRS-GFP lentiviral vectors and used to transduce SSCs, with the expression of the exogenous proteins confirmed by Western blot. For gene transfection, COS-1 cells or osteoblast progenitors were transfected with Lipofectamine 2000 (Invitrogen). For reporter assays, COS-1 cells were transfected with expression vectors and either 8xGTIIC or 6xOSE reporters. Luciferase activity in cell lysates was determined as previously described 9 (link).
Tang Y., Feinberg T., Keller E.T., Li X.Y, & Weiss S.J. (2016). Snail/Slug-YAP/TAZ Complexes Control Skeletal Stem Cell Self-Renewal and Differentiation. Nature cell biology, 18(9), 917-929.
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2

Silencing TEAD and SNAIL/SLUG genes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human or mouse TEF-1, TEF-3, TEF-4 and TEF-5 (TEAD1–4) siRNAs, and human SNAIL or SLUG siRNAs were obtained from Santa Cruz Technology. To silence gene expression, PepMute siRNA Transfection Reagent (SignaGen Laboratories) was used for all siRNAs transfection. Flag-Snail, Snail-HA, Slug-Myc, Flag-YAP, Flag-TAZ, and 8xGTIIC were obtained from Addgene. HA-Runx2 and 6xOSE reporter constructs were provided by G. Stein 16 (link). Snail, Slug, YAP and TAZ mutants were constructed by PCR. Where indicated, Flag-YAP and Flag-TAZ were cloned into pLentilox-IRS-GFP lentiviral vectors and used to transduce SSCs, with the expression of the exogenous proteins confirmed by Western blot. For gene transfection, COS-1 cells or osteoblast progenitors were transfected with Lipofectamine 2000 (Invitrogen). For reporter assays, COS-1 cells were transfected with expression vectors and either 8xGTIIC or 6xOSE reporters. Luciferase activity in cell lysates was determined as previously described 9 (link).
Tang Y., Feinberg T., Keller E.T., Li X.Y, & Weiss S.J. (2016). Snail/Slug-YAP/TAZ Complexes Control Skeletal Stem Cell Self-Renewal and Differentiation. Nature cell biology, 18(9), 917-929.
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