For FACS of B cells, PBMC were stained with BUV395 anti-CD19 (BD Biosciences), APC anti-CD27 (Biolegend) and either PE anti-CXCR3 (eBioscience), PE-Cy7 anti-CXCR5 (eBioscience), PE anti-CD11a (Biolegend), PE anti-CD49d (Biolegend), PE anti-CD166 (BD) or FITC anti-CD6 (Biolegend). BUV395 anti-CD19 APC anti-CD27-stained cells were also fixed and permeabilized and stained with rabbit anti-AHNAK (Sigma) and PE donkey anti-rabbit IgG. FCS files of stained cells were collected on a LSRII cytometer (BD) and analyzed using FloJo.
Buv395 anti cd19 apc anti cd27
Manufactured by Merck Group
BUV395 anti-CD19 APC anti-CD27 is a fluorochrome-conjugated antibody used for flow cytometry. It binds to the CD19 and CD27 cell surface markers, which are expressed on specific cell types. The core function of this product is to enable the detection and analysis of these cell populations in research applications.
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Lab products found in correlation
Buv395 anti cd19, by BD (2 mentions)
Anti cd27 apc, by BioLegend (2 mentions)
Pe anti cxcr3, by Thermo Fisher Scientific (2 mentions)
Pe cy7 anti cxcr5, by Thermo Fisher Scientific (2 mentions)
Anti cd166 pe, by BD (2 mentions)
Fitc anti cd6, by BioLegend (2 mentions)
Rabbit anti ahnak, by Merck Group (2 mentions)
Pe anti cd11a, by BioLegend (2 mentions)
Anti cd49d pe, by BioLegend (2 mentions)
Lsrii cytometer, by BD (2 mentions)
2 protocols using buv395 anti cd19 apc anti cd27
1
FACS Analysis of B Cell Subsets
2
FACS Analysis of B Cell Subsets
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For FACS of B cells, PBMC were stained with BUV395 anti-CD19 (BD Biosciences), APC anti-CD27 (Biolegend) and either PE anti-CXCR3 (eBioscience), PE-Cy7 anti-CXCR5 (eBioscience), PE anti-CD11a (Biolegend), PE anti-CD49d (Biolegend), PE anti-CD166 (BD) or FITC anti-CD6 (Biolegend). BUV395 anti-CD19 APC anti-CD27-stained cells were also fixed and permeabilized and stained with rabbit anti-AHNAK (Sigma) and PE donkey anti-rabbit IgG. FCS files of stained cells were collected on a LSRII cytometer (BD) and analyzed using FloJo.
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