Quantitative measurement of tissue nonheme iron was performed using the method of Torrance and Bothwelld [27 (link), 34 ]. The results are presented as micrograms of iron per gram wet weight of tissue. Serum iron concentrations were determined using a serum iron assay kit (Nanjing Jiancheng Bioengineering institute, Nanjing, China, Catalog A040-1-1). Total iron binding capacity (TIBC) was determined using a total iron binding capacity assay kit (Nanjing Jiancheng Bioengineering institute, Nanjing, China, Catalog A039-1-1), according to the manufacturer’s instructions. Transferrin saturation was directly calculated from the serum iron and TIBC.
Total iron binding capacity assay kit
Manufactured by Nanjing Jiancheng
Sourced in China
The Total Iron Binding Capacity Assay Kit is a laboratory tool designed to measure the total iron binding capacity in a sample. It quantifies the maximum amount of iron that can be bound by the iron-binding proteins present in the sample.
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Lab products found in correlation
2 protocols using total iron binding capacity assay kit
1
Quantitative Tissue Iron Measurement
2
Serum Iron and Binding Capacity Assay
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Serum iron content was measured using serum iron assay kit (Jiancheng, Nanjing, China) according to the manufacturer's instructions. Total iron binding capacity was measured using total iron binding capacity assay kit (Jiancheng, Nanjing, China) according to the manufacturer's instructions. Optical density was measured absorbance at 520 nm by a microplate reader (Molecular Devices, Sunnyvale, USA). And transferrin saturation (TSAT, %) = serum iron (mg/L)/total iron binding capacity (mg/L) × 100.
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