PeroxyFluor-1 (PF-1)42 (link) and 2-acetylbenzofuran (2-ABF)35 (link) were synthesized according to the reported procedures. Kanamycin, isopropyl-β-d-thiogalactopyranoside (IPTG), and 5-aminolevulinic acid were purchased from commercial suppliers. Lysozyme, DNase, and protein inhibitor cocktail (PI cocktail) were purchased from Roche Diagnostics. Spinach ferredoxin (SFdx) and spinach ferredoxin reductase (SFdR) were purchased from Sigma Aldrich.
Spinach ferredoxin reductase
Manufactured by Merck Group
Spinach ferredoxin reductase is an enzyme found in the chloroplasts of spinach plants. It catalyzes the transfer of electrons from NADPH to ferredoxin, a key step in the photosynthetic electron transport chain. The enzyme plays a crucial role in the regulation of photosynthesis and other metabolic processes in plants.
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Lab products found in correlation
2 protocols using spinach ferredoxin reductase
1
Synthesis and Reagent Acquisition for Fluorescent Assay
2
C. thermocellum Fatty Aldehydes Conversion
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Extracts containing fatty aldehydes from C. thermocellum were mixed with 130 μM purified SeADO, 80 μM Fe(NH 4 ) 2 (SO 4 ) 2 , 10 μM spinach ferredoxin (Sigma-Aldrich), 1.8 μM spinach ferredoxin reductase (Sigma-Aldrich) and 2 mM NADPH in 100 μL of 100 mM HEPES buffer (pH 7.2) containing 0.1 M KCl and 10% glycerol. The reactions were incubated at 25 • C for 3 h, quenched by the addition of 100 μL of ethyl acetate, and mixed using a vortex mixer to extract the hydrocarbon product and unreacted substrate. A 1 μL aliquot of the ethyl acetate layer was injected into the GC-MS instrument (Zhang, Lu and Li 2013) . The parameters for GC-MS were as described previously (Schirmer et al. 2010) .
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