Blood levels of hsCRP were analyzed using an immunoturbidimetric assay (Siemens, Healthineers). The limit of quantification was 3 mg/L. The analysis was carried out by a commercial laboratory (Unilabs AB, Stockholm, Sweden). MCP‐1 levels were analyzed using commercial electrochemiluminescence enzyme‐linked immunosorbent assays (ELISA; Human MCP‐1 Ultra‐Sensitive Kit), and sCD14 and YKL‐40 were analyzed using commercial colorimetric ELISAs (Human sCD14 quantikine ELISA kit, Human chitinase‐3 quantikine ELISA kit, R&D Systems Inc.) at the Clinical Neurochemistry Laboratory in Mölndal, Sweden. Intra‐assay coefficients of variation were below 10% for all assays. The staff performing the analyses were blinded, to patient identity and diagnosis (Jakobsson et al., 2015 (link)).
Human chitinase 3 quantikine elisa kit
Manufactured by R&D Systems
Sourced in Sweden
The Human chitinase-3 Quantikine ELISA Kit is a quantitative sandwich enzyme immunoassay designed to measure human chitinase-3 levels in cell culture supernates, serum, and plasma. The assay employs an antibody specific for human chitinase-3 coated on a microplate. Standards and samples are pipetted into the wells, and any human chitinase-3 present is bound by the immobilized antibody.
Automatically generated - may contain errors
Lab products found in correlation
Nf light elisa, by UmanDiagnostics (3 mentions)
Human scd14 quantikine elisa kit, by R&D Systems (2 mentions)
Immunoturbidimetric assay, by Siemens (1 mentions)
Inno bia alzbio3 kit, by Fujirebio (1 mentions)
Human mcp 1 ultra sensitive kit, by Mesoscale (1 mentions)
Innotest elisa, by Fujirebio (1 mentions)
6 protocols using human chitinase 3 quantikine elisa kit
1
Biomarker Analyses in Clinical Study
2
Comprehensive Biomarker Analysis in CSF
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The CSF concentrations of MCP-1, sAPP-α and sAPP-β, and AβX-38, AβX-40, and AβX-42 were determined using the MSD® Human MCP-1 Ultra-Sensitive Kit, MSD® sAPP-α/sAPP-β Multiplex Assay, and MSD ® Human/Rodent (4G8) Abeta-Triplex Assay, respectively, as described by the manufacturer (Meso Scale Discovery, Gaithersburg, MD, USA). CSF concentrations of P-tau, T-tau, and Aβ1-42 were measured simultaneously by the Luminex xMAP technology using the Inno-Bia AlzBio3 kit (Innogenetics, Zwijndrecht, Belgium). The MSD-derived Aβ concentrations were derived using a detection antibody against the mid-domain of the Aβ proteins, whereas we also measured Aβ-42 using the AlzBio3 kit. This kit includes a neo-epitope-specific antibody against the first amino acids of Aβ. For this reason, we denote MSD-derived Aβ concentrations AβX-38, AβX-40 and AβX-42 and AlzBio3-derived Aβ concentrations Aβ1-42 throughout the manuscript. sCD14 and YKL-40 were determined by Human sCD14 quantikine ELISA kit and Human chitinase-3 quantikine ELISA kit, respectively (R&D systems, Inc, Minneapolis, MN). All CSF analyses were performed in one round of analyses using one batch of reagents by board-certified laboratory technicians who were blinded to clinical information.
3
CSF Biomarker Measurements in EMIF-AD MDB
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Details of the CSF biomarker measurements can be found in Bos et al. 4 In brief, the CSF specimens were collected individually at each of the 11 EMIF-AD MDB participating sites. CSF samples were shipped to the Department of Psychiatry and Neurochemistry at University of Gothenburg, Sweden. Relevant to the analyses presented here, NfL levels were measured using a commercial enzyme-linked immunosorbent assay (ELISA; NF-light ELISA, UmanDiagnostics; Zetterberg et al. 18 ).
Ng levels were measured using an in-house immunoassay for Ng. 19 YKL-40 levels were measured using a human chitinase-3 quantikine ELISA kit (R&D Systems, Inc.; Olsson et al. 20 ). To reduce the skewness of phenotype distributions, data for all three CSF variables were log-transformed prior to analysis (Figure S1 in supporting information).
Ng levels were measured using an in-house immunoassay for Ng. 19 YKL-40 levels were measured using a human chitinase-3 quantikine ELISA kit (R&D Systems, Inc.; Olsson et al. 20 ). To reduce the skewness of phenotype distributions, data for all three CSF variables were log-transformed prior to analysis (Figure S1 in supporting information).
4
CSF Biomarker Measurement Protocols
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Details of the CSF biomarker measurements can be found in Bos et al. 4 . In brief, the CSF specimen were collected individually at each of the 11 EMIF-AD MDB participating sites. CSF samples were shipped to Department of Psychiatry and Neurochemistry at University of Gothenburg, Sweden.
was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
Relevant to the analyses presented here, NF-L levels were measured using a commercial ELISA (NFlight ELISA, UmanDiagnostics, Umeå, Sweden 18 ). Ng levels were measured using an in-house immunoassay for Ng 19 (link) . YKL-40 levels were measured using a human chitinase-3 quantikine ELISA kit (R&D systems, Inc, Minneapolis, MN 20 ). To reduce the skewness of phenotype distributions, data for all three CSF variables were log-transformed prior to analysis (Supplementary Figure 1).
was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
Relevant to the analyses presented here, NF-L levels were measured using a commercial ELISA (NFlight ELISA, UmanDiagnostics, Umeå, Sweden 18 ). Ng levels were measured using an in-house immunoassay for Ng 19 (link) . YKL-40 levels were measured using a human chitinase-3 quantikine ELISA kit (R&D systems, Inc, Minneapolis, MN 20 ). To reduce the skewness of phenotype distributions, data for all three CSF variables were log-transformed prior to analysis (Supplementary Figure 1).
5
Multiplex Cytokine and Biomarker Assays
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IL-8 was analyzed together with IL-1β, IL-2, IL-4, IL-5, IL-10, IL-12, IL-13, TNF-α, and IFN-γ using the MSD 96-well multi-array and multi-spot human cytokine assay (Human Cytokine Assay Ultra-Sensitive kit, Meso Scale Discovery). MCP-1 concentration was measured using a commercial electrochemiluminescence enzymelinked immunosorbant assay (ELISA; Human MCP-1 Ultra-Sensitive Kit, Meso Scale Discovery). YKL-40 concentration was measured using a commercial colorimetric ELISA (Human chitinase-3 quantikine ELISA kit, R&D systems Inc.). NF-L concentration was measured with a commercial ELISA assay (NFLight, UmanDiagnostis AB, Umeå, Sweden).
All analyses were performed according to the manufacturers' instructions at the Clinical Neurochemistry Laboratory in Mölndal, Sweden. Intra-assay coefficients of variation were below 10% for all assays. The staff performing the analyses was blinded to all phenotype information.
All analyses were performed according to the manufacturers' instructions at the Clinical Neurochemistry Laboratory in Mölndal, Sweden. Intra-assay coefficients of variation were below 10% for all assays. The staff performing the analyses was blinded to all phenotype information.
6
Cerebrospinal Fluid Biomarker Analysis
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Central CSF analyses were conducted at Gothenburg University, Sweden. NFL concentrations were measured using a commercial ELISA (NF-light ELISA, UmanDiagnostics, Ume a, Sweden [7] ). Ng was measured using an in-house immunoassay for Ng [10] . YKL-40 was determined by a human chitinase-3 quantikine ELISA kit (R&D systems, Inc, Minneapolis, MN [27] ). Ab 38 , Ab 40 , and Ab 42 were measured using the V-PLEX Plus Ab Peptide Panel 1 (6E10) Kit from Meso Scale Discovery (MSD, Rockville, MD). All analyses were performed according to the manufacturer's instructions by board-certified laboratory technicians who were blinded to clinical information. All measurements were performed on one occasion using one batch of reagents, except for n 5 8 samples from the EDAR cohort that were analyzed beforehand in the same laboratory, but in a different batch. For P-tau and T-tau, we used available measures from the local cohorts (P-tau n 5 630; T-tau n 5 621) derived in clinical laboratory practice using INNOTEST ELISAs (Fujirebio, Ghent, Belgium).
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