Cells were cultured under standard cell culture incubator conditions at 37 °C in a humidified atmosphere containing 5% CO2 and were used from passages 3–7. MDA-MB-231 cells were maintained in RPMI 1640 medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS, Sigma-Aldrich,). shCOX2-MDA-MB-231 cells were maintained in RPMI 1640 medium supplemented with 10% FBS and G418 sulfate (Corning, Corning, NY, USA) at a concentration of 400 μg/mL. SUM-149 cells were maintained in DMEM/Ham’s F12 50/50 medium (Sigma-Aldrich) with 5% calf serum, insulin (5 μg/ml), and hydrocortisone (1 μg/mL). Pa09C cells were cultured in RPMI-1640 with 20% FBS, 12.5 mmol/L glucose, and 2 mmol/L glutamine. Pa20C cells were cultured in DMEM (Sigma-Aldrich) with 10% FBS, 25 mmol/L glucose, and 4 mmol/L glutamine.
Dmem ham s f12 50 50 medium
Manufactured by Merck Group
Sourced in United States
DMEM/Ham's F12 50/50 medium is a cell culture medium that provides a balanced combination of essential nutrients for the growth and maintenance of a variety of cell types. It is a 1:1 mixture of Dulbecco's Modified Eagle's Medium (DMEM) and Ham's F12 medium, providing a versatile and widely-used formulation for diverse cell culture applications.
Automatically generated - may contain errors
2 protocols using dmem ham s f12 50 50 medium
1
Cell Culture Conditions for MDA-MB-231, shCOX2-MDA-MB-231, SUM-149, Pa09C, and Pa20C
2
Cell Culture Conditions Across Diverse Cell Lines
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Cells were cultured under standard cell culture incubator conditions at 37 o C in a humidi ed atmosphere containing 5% CO 2 and were used from passages 3-7. MDA-MB-231 cells were maintained in RPMI 1640 medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS, Sigma-Aldrich,). shCOX2-MDA-MB-231 cells were maintained in RPMI 1640 medium supplemented with 10% FBS and G418 sulfate (Corning, Corning, NY, USA) at a concentration of 400 μg/ml. SUM-149 cells were maintained in DMEM/Ham's F12 50/50 medium (Sigma-Aldrich) with 5% calf serum, insulin (5 μg/ml), and hydrocortisone (1 μg/ml). Pa09C cells were cultured in RPMI-1640 with 20% FBS, 12.5 mmol/L glucose, and 2 mmol/L glutamine. Pa20C cells were cultured in DMEM (Sigma-Aldrich) with 10% FBS, 25 mmol/L glucose, and 4 mmol/L glutamine.
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