The tetrazolium dye, MTT, is widely used to assess the viability or the metabolic state of the cells. The MTT-colorimetric monocyte mediated cytotoxicity assay is based on the ability of living cells that reduce MTT into formazan by mitochondrial succinate dehydrogenase in viable cells. After treatment at the different culture conditions, Jurkat cells were plated in 96-well at-bottom tissue culture plates to attain a nal concentration of 2 × 10 6 cells/mL. After incubation for 12 hours at 37 o C, the resultant Jurkat cell viability was determined by the MTT viability assay (ATCC, Manassas, VA, USA)
Mtt viability assay
Manufactured by American Type Culture Collection
Sourced in United States
The MTT viability assay is a colorimetric assay that measures the metabolic activity of cells. It is used to assess cell viability, proliferation, and cytotoxicity. The assay utilizes the tetrazolium salt MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) which is reduced by metabolically active cells to form a purple formazan product that can be quantified spectrophotometrically.
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3 protocols using mtt viability assay
1
Evaluating Jurkat Cell Viability via MTT Assay
2
Assessing Jurkat Cell Viability via MTT Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The tetrazolium dye, MTT, is widely used to assess the viability or the metabolic state of the cells. The MTT-colorimetric monocyte mediated cytotoxicity assay, is based on the ability of living cells to reduce MTT into formazan by mitochondrial succinate dehydrogenase in viable cells. After treatment at the different culture conditions, Jurkat cells were plated in 96-well flat-bottom tissue culture plates to attain a final concentration of 2 × 10 6 cells/mL. After incubation for 12 hours at 37 • C, the resultant Jurkat cell viability was determined by the MTT viability assay (ATCC, Manassas, VA, USA).
3
Evaluating Jurkat Cell Viability Using MTT Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The tetrazolium dye, MTT, is widely used to assess the viability or the metabolic state of the cells. The MTT-colorimetric monocyte mediated cytotoxicity assay, is based on the ability of living cells to reduce MTT into formazan by mitochondrial succinate dehydrogenase in viable cells. After treatment at the different culture conditions, Jurkat cells were plated in 96-well at-bottom tissue culture plates to attain a nal concentration of 2 × 10 6 cells/mL. After incubation for 12 hours at 37 o C, the resultant Jurkat cell viability was determined by the MTT viability assay (ATCC, Manassas, VA, USA)
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